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NDRG4 antibody

Anti-NDRG4 antibody used in Western Blot (WB). GTX118019
Anti-NDRG4 antibody used in IHC (Paraffin sections) (IHC-P). GTX118019

Cat No. GTX118019

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P

Reactivity

Human
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

38 kDa. ( Note )

Positive Control

293T , Jurkat

Predict Reactivity

Mouse, Rat, Zebrafish, Bovine, Xenopus laevis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human NDRG4. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11166938

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

NDRG family member 4 , BDM1 , SMAP-8 , SMAP8

Background

This gene is a member of the N-myc downregulated gene family which belongs to the alpha/beta hydrolase superfamily. The protein encoded by this gene is a cytoplasmic protein that may be involved in the regulation of mitogenic signalling in vascular smooth muscles cells. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-NDRG4 antibody used in Western Blot (WB). GTX118019

GTX118019 WB Image

Sample (30 ug of whole cell lysate)
A: Jurakt
10% SDS PAGE
GTX118019 diluted at 1:1000

Anti-NDRG4 antibody used in IHC (Paraffin sections) (IHC-P). GTX118019

GTX118019 IHC-P Image

NDRG4 antibody detects NDRG4 protein at cytoplasm on human hepatoma by immunohistochemical analysis.
Sample: Paraffin-embedded hepatoma.
NDRG4 antibody (GTX118019) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf