GeneTex
United States (US)

NDUFA10 antibody [N1C3]

GTX103093_39785_IHC_19011506_625.jpg
GTX103093_WB_19011506_387.jpg
GTX103093_39785_20161013_WB_M_kidney_19011506_112.jpg
GTX103093_39785_IFA_19011506_849.jpg

Cat No. GTX103093

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

41 kDa. ( Note )

Positive Control

293T , A431 , H1299 , HeLaS3 , HepG2 , Molt-4 , Raji , mouse kidney

Predict Reactivity

Bovine, Chimpanzee(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.82mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human NDUFA10. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

CI-42KD antibody, CI-42k antibody, FLJ21923 antibody, MGC5103 antibody, NDUFA10 antibody, complex I 42kDa subunit antibody, "NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, mitochondrial antibody", NADH-ubiquinone oxidoreductase 42 kDa subunit antibody, "NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 10, 42kDa antibody"

Cellular Localization

Mitochondrion matrix

Background

The protein encoded by this gene belongs to the complex I 42kDA subunit family. Mammalian complex I is the first enzyme complex in the electron transport chain of mitochondria. It is composed of 45 different subunits. This protein is a component of the hydrophobic protein fraction and has NADH dehydrogenase activity and oxidoreductase activity. It transfers electrons from NADH to the respiratory chain. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX103093_39785_IHC_19011506_625.jpg

GTX103093 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human colon , using NDUFA10(GTX103093) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX103093_WB_19011506_387.jpg

GTX103093 WB Image

Sample(30 μg of whole cell lysate)
A:H1299
B:Raji(GTX27908)
10% SDS PAGE
GTX103093 diluted at 1:500

GTX103093_39785_20161013_WB_M_kidney_19011506_112.jpg

GTX103093 WB Image

Mouse tissue extract (50 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with NDUFA10 antibody [N1C3] (GTX103093) diluted at 1:500.

GTX103093_39785_IFA_19011506_849.jpg

GTX103093 ICC/IF Image

NDUFA10 antibody [N1C3] detects NDUFA10 protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 2% paraformaldehyde/culture medium at 37℃ for 30 min.
Green: NDUFA10 protein stained by NDUFA10 antibody [N1C3] (GTX103093) diluted at 1:500.
Blue: Hoechst 33342 staining.

SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169