APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Dilution |
| 1:500-1:3000 |
| 1:100-1:1000 |
| 1:100-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
293T , A431 , H1299 , HeLaS3 , HepG2 , Molt-4 , Raji , mouse kidney
Predict Reactivity
Bovine, Chimpanzee(>80% identity)
PROPERTIES
Form
Liquid
Buffer
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.82mg/ml(Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human NDUFA10. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
CI-42KD antibody, CI-42k antibody, FLJ21923 antibody, MGC5103 antibody, NDUFA10 antibody, complex I 42kDa subunit antibody, "NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, mitochondrial antibody", NADH-ubiquinone oxidoreductase 42 kDa subunit antibody, "NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 10, 42kDa antibody"
Cellular Localization
Mitochondrion matrix
Background
The protein encoded by this gene belongs to the complex I 42kDA subunit family. Mammalian complex I is the first enzyme complex in the electron transport chain of mitochondria. It is composed of 45 different subunits. This protein is a component of the hydrophobic protein fraction and has NADH dehydrogenase activity and oxidoreductase activity. It transfers electrons from NADH to the respiratory chain. [provided by RefSeq]
Database
Research Area
DATA IMAGES
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GTX103093 IHC-P Image
Immunohistochemical analysis of paraffin-embedded human colon , using NDUFA10(GTX103093) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX103093 WB Image
Sample(30 μg of whole cell lysate)
A:H1299
B:Raji(GTX27908)
10% SDS PAGE
GTX103093 diluted at 1:500
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GTX103093 WB Image
Mouse tissue extract (50 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with NDUFA10 antibody [N1C3] (GTX103093) diluted at 1:500.
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GTX103093 ICC/IF Image
NDUFA10 antibody [N1C3] detects NDUFA10 protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 2% paraformaldehyde/culture medium at 37℃ for 30 min.
Green: NDUFA10 protein stained by NDUFA10 antibody [N1C3] (GTX103093) diluted at 1:500.
Blue: Hoechst 33342 staining.
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