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NDUFA10 antibody

Anti-NDUFA10 antibody used in Western Blot (WB). GTX114572
Anti-NDUFA10 antibody used in Western Blot (WB). GTX114572
Anti-NDUFA10 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114572
Anti-NDUFA10 antibody used in IHC (Paraffin sections) (IHC-P). GTX114572

Cat. No. GTX114572

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all NDUFA10 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

41 kDa. ( Note )

Positive Control

mouse kidney , Jurkat

Predict Reactivity

Bovine, Chimpanzee(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human NDUFA10. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10619603

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

NADH:ubiquinone oxidoreductase subunit A10 , CI-42KD , CI-42k , MC1DN22

Cellular Localization

Mitochondrion matrix

Background

The protein encoded by this gene belongs to the complex I 42kDA subunit family. Mammalian complex I is the first enzyme complex in the electron transport chain of mitochondria. It is composed of 45 different subunits. This protein is a component of the hydrophobic protein fraction and has NADH dehydrogenase activity and oxidoreductase activity. It transfers electrons from NADH to the respiratory chain. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-NDUFA10 antibody used in Western Blot (WB). GTX114572

GTX114572 WB Image

NDUFA10 antibody detects NDUFA10 protein by Western blot analysis.
A. 50 μg mouse kidney lysate/extract
10% SDS-PAGE
NDUFA10 antibody (GTX114572) dilution: 1:1000

Anti-NDUFA10 antibody used in Western Blot (WB). GTX114572

GTX114572 WB Image

Sample (30 ug of whole cell lysate)
A: JurKat
10% SDS PAGE
GTX114572 diluted at 1:1000

Anti-NDUFA10 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114572

GTX114572 ICC/IF Image

Immunofluorescence analysis of methanol-fixed A431, using NDUFA10(GTX114572) antibody at 1:200 dilution.

Anti-NDUFA10 antibody used in IHC (Paraffin sections) (IHC-P). GTX114572

GTX114572 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human breast cancer, using NDUFA10(GTX114572) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

Application Reference

David Molina-Granada et al. Commun Biol 2022; 5 (1) : 620 Most mitochondrial dGTP is tightly bound to respiratory complex I through the NDUFA10 subunit.
Application : WB
Reactivity : Human, Mouse
Garcia-Esparcia P et al. Brain Pathol 2016; (Epub) Mitochondrial activity in the frontal cortex area 8 and angular gyrus in Parkinson's disease and Parkinson's disease with dementia.
Application : WB
Reactivity : Human
Submit a Reference

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169