Mouse tissue extract (50 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with NDUFA5 antibody [N1C3] (GTX111016) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with NDUFA5 antibody [N1C3] (GTX111016) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Sample (30 ug of whole cell lysate)
A: Hep G2 (GTX27900)
15% SDS PAGE
GTX111016 diluted at 1:1000

Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using NDUFA5(GTX111016) antibody at 1:500 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

NDUFA5 antibody [N1C3] detects NDUFA5 protein at mitochondria in human colon carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human colon carcinoma.
NDUFA5 antibody [N1C3] (GTX111016) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min