APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1:500 - 1:2000 |
1:50 - 1:100 |
1:50 - 1:100 |
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
PBS, 50% Glycerol
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
Batch dependent (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-116 of human NDUFA5 (NP_004991.1).
Purification
Purified by affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
NADH:ubiquinone oxidoreductase subunit A5 , B13 , CI-13KD-B , CI-13kB , NUFM , UQOR13
Cellular Localization
Mitochondrion inner membrane
Background
This nuclear gene encodes a conserved protein that comprises the B13 subunit of complex I of the mitochondrial respiratory chain. The encoded protein localizes to the inner mitochondrial membrane, where it is thought to aid in the transfer of electrons from NADH to ubiquinone. Alternative splicing results in multiple transcript variants. There are numerous pseudogenes of this gene on chromosomes 1, 3, 6, 8, 9, 11, 12, and 16. [provided by RefSeq, Apr 2014]
Database
Research Area
DATA IMAGES
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GTX35222 WB Image
WB analysis of normal (control) and knockout (KO) HeLa cell lysate using GTX35222 NDUFA5 antibody. Dilution : 1:1000 Loading : 25μg per lane
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GTX35222 IHC-P Image
IHC-P analysis of human gastric cancer tissue using GTX35222 NDUFA5 antibody. Dilution : 1:100
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GTX35222 IHC-P Image
IHC-P analysis of human stomach tissue using GTX35222 NDUFA5 antibody. Dilution : 1:100
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GTX35222 ICC/IF Image
ICC/IF analysis of NIH/3T3 cells using GTX35222 NDUFA5 antibody. Blue : DAPI Dilution : 1:100
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GTX35222 IHC-P Image
IHC-P analysis of human tonsil tissue using GTX35222 NDUFA5 antibody. Dilution : 1:100
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REFERENCE
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REVIEW
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