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NDUFA5 antibody

KO/KD
Anti-NDUFA5 antibody used in Western Blot (WB). GTX35222
Anti-NDUFA5 antibody used in IHC (Paraffin sections) (IHC-P). GTX35222
Anti-NDUFA5 antibody used in IHC (Paraffin sections) (IHC-P). GTX35222
Anti-NDUFA5 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX35222
Anti-NDUFA5 antibody used in IHC (Paraffin sections) (IHC-P). GTX35222

Cat. No. GTX35222

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399)
See all NDUFA5 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:100
IHC-P 1:50 - 1:100
Not tested in other applications.

Calculated MW

13 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-116 of human NDUFA5 (NP_004991.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

NADH:ubiquinone oxidoreductase subunit A5 , B13 , CI-13KD-B , CI-13kB , NUFM , UQOR13

Cellular Localization

Mitochondrion inner membrane

Background

This nuclear gene encodes a conserved protein that comprises the B13 subunit of complex I of the mitochondrial respiratory chain. The encoded protein localizes to the inner mitochondrial membrane, where it is thought to aid in the transfer of electrons from NADH to ubiquinone. Alternative splicing results in multiple transcript variants. There are numerous pseudogenes of this gene on chromosomes 1, 3, 6, 8, 9, 11, 12, and 16. [provided by RefSeq, Apr 2014]

Database

Research Area

DATA IMAGES

Anti-NDUFA5 antibody used in Western Blot (WB). GTX35222

GTX35222 WB Image

KO/KD

WB analysis of normal (control) and knockout (KO) HeLa cell lysate using GTX35222 NDUFA5 antibody.
Dilution : 1:1000
Loading : 25μg per lane

Anti-NDUFA5 antibody used in IHC (Paraffin sections) (IHC-P). GTX35222

GTX35222 IHC-P Image

IHC-P analysis of human gastric cancer tissue using GTX35222 NDUFA5 antibody.
Dilution : 1:100

Anti-NDUFA5 antibody used in IHC (Paraffin sections) (IHC-P). GTX35222

GTX35222 IHC-P Image

IHC-P analysis of human stomach tissue using GTX35222 NDUFA5 antibody.
Dilution : 1:100

Anti-NDUFA5 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX35222

GTX35222 ICC/IF Image

ICC/IF analysis of NIH/3T3 cells using GTX35222 NDUFA5 antibody.
Blue : DAPI
Dilution : 1:100

Anti-NDUFA5 antibody used in IHC (Paraffin sections) (IHC-P). GTX35222

GTX35222 IHC-P Image

IHC-P analysis of human tonsil tissue using GTX35222 NDUFA5 antibody.
Dilution : 1:100

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399