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NDUFA8 antibody

Anti-NDUFA8 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX65622
Anti-NDUFA8 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX65622
Anti-NDUFA8 antibody used in Immunoprecipitation (IP). GTX65622
Anti-NDUFA8 antibody used in Western Blot (WB). GTX65622

Cat. No. GTX65622

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IP

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399)
See all NDUFA8 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:200
IP 1:50 - 1:100
Not tested in other applications.

Calculated MW

20 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 33-172 of human NDUFA8 (NP_055037.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

NADH:ubiquinone oxidoreductase subunit A8 , CI-19KD , CI-PGIV , PGIV

Cellular Localization

Mitochondrion inner membrane,Mitochondrion

Background

The protein encoded by this gene belongs to the complex I 19 kDa subunit family. Mammalian complex I is composed of 45 different subunits. This protein has NADH dehydrogenase activity and oxidoreductase activity. It plays an important role in transfering electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. Alternative splicing of this gene results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Dec 2015]

Database

Research Area

DATA IMAGES

Anti-NDUFA8 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX65622

GTX65622 ICC/IF Image

ICC/IF analysis of H9C2 cells using GTX65622 NDUFA8 antibody.
Blue : DAPI
Dilution : 1:100

Anti-NDUFA8 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX65622

GTX65622 ICC/IF Image

ICC/IF analysis of L929 cells using GTX65622 NDUFA8 antibody.
Blue : DAPI
Dilution : 1:100

Anti-NDUFA8 antibody used in Immunoprecipitation (IP). GTX65622

GTX65622 IP Image

IP analysis of 293T cell lysate using GTX65622 NDUFA8 antibody.
Antibody amount : 3μg / 200μg lysate
Dilution : 1:1000

Anti-NDUFA8 antibody used in Western Blot (WB). GTX65622

GTX65622 WB Image

WB analysis of various sample lysates using GTX65622 NDUFA8 antibody.
Dilution : 1:3000
Loading : 25μg per lane

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399