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NDUFAB1 antibody

Anti-NDUFAB1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX111875
Anti-NDUFAB1 antibody used in Western Blot (WB). GTX111875
Anti-NDUFAB1 antibody used in IHC (Paraffin sections) (IHC-P). GTX111875
Anti-NDUFAB1 antibody used in IHC (Paraffin sections) (IHC-P). GTX111875
Anti-NDUFAB1 antibody used in IHC (Paraffin sections) (IHC-P). GTX111875
Anti-NDUFAB1 antibody used in IHC (Paraffin sections) (IHC-P). GTX111875
Anti-NDUFAB1 antibody used in Western Blot (WB). GTX111875

Cat No. GTX111875

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse, Rat
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

17 kDa. ( Note )

Positive Control

GL261 , C8D30 , PC-12

Predict Reactivity

Bovine, Pig, Xenopus laevis, Chimpanzee, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human NDUFAB1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

NADH:ubiquinone oxidoreductase subunit AB1,ACP,ACP1,FASN2A,SDAP

Cellular Localization

Mitochondrion

Background

Carrier of the growing fatty acid chain in fatty acid biosynthesis in mitochondria. Accessory and non-catalytic subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), which functions in the transfer of electrons from NADH to the respiratory chain.

Database

Research Area

DATA IMAGES
Anti-NDUFAB1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX111875

GTX111875 ICC/IF Image

Immunofluorescence analysis of methanol-fixed A431, using NDUFAB1(GTX111875) antibody at 1:200 dilution.

Anti-NDUFAB1 antibody used in Western Blot (WB). GTX111875

GTX111875 WB Image

NDUFAB1 antibody detects NDUFAB1 protein by Western blot analysis.
A. 30 µg GL261 whole cell lysate/extract
B. 30 ug C8D30 whole cell lysate/extract
12 % SDS-PAGE
NDUFAB1 antibody (GTX111875) dilution: 1:5000

Anti-NDUFAB1 antibody used in IHC (Paraffin sections) (IHC-P). GTX111875

GTX111875 IHC-P Image

NDUFAB1 antibody detects NDUFAB1 protein at mitochondria on mouse pancreas by immunohistochemical analysis.
Sample: Paraffin-embedded mouse pancreas.
NDUFAB1 antibody (GTX111875) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-NDUFAB1 antibody used in IHC (Paraffin sections) (IHC-P). GTX111875

GTX111875 IHC-P Image

NDUFAB1 antibody detects NDUFAB1 protein at mitochondria in mouse duodenum by immunohistochemical analysis.
Sample: Paraffin-embedded mouse duodenum.
NDUFAB1 antibody (GTX111875) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-NDUFAB1 antibody used in IHC (Paraffin sections) (IHC-P). GTX111875

GTX111875 IHC-P Image

NDUFAB1 antibody detects NDUFAB1 protein at mitochondria on mouse intestine by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
NDUFAB1 antibody (GTX111875) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-NDUFAB1 antibody used in IHC (Paraffin sections) (IHC-P). GTX111875

GTX111875 IHC-P Image

NDUFAB1 antibody detects NDUFAB1 protein at mitochondria in mouse brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
NDUFAB1 antibody (GTX111875) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-NDUFAB1 antibody used in Western Blot (WB). GTX111875

GTX111875 WB Image

NDUFAB1 antibody detects NDUFAB1 protein by Western blot analysis.
A. 30 µg PC-12 whole cell lysate/extract
12 % SDS-PAGE
NDUFAB1 antibody (GTX111875) dilution: 1:5000

REFERENCE
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REVIEW
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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169