Immunohistochemical analysis of paraffin-embedded human breast cancer, using NDUFB10(GTX114576) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Immunofluorescence analysis of methanol-fixed A431, using NDUFB10(GTX114576) antibody at 1:200 dilution.

Mouse tissue extract (50 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with NDUFB10 antibody [N1C3] (GTX114576) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Whole cell extract (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with NDUFB10 antibody [N1C3] (GTX114576) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.