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NDUFB4 antibody

Anti-NDUFB4 antibody used in IHC (Paraffin sections) (IHC-P). GTX111026
Anti-NDUFB4 antibody used in Western Blot (WB). GTX111026
Anti-NDUFB4 antibody used in IHC (Paraffin sections) (IHC-P). GTX111026
Anti-NDUFB4 antibody used in IHC (Paraffin sections) (IHC-P). GTX111026

Cat. No. GTX111026

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Rat
Package
100 μl ($399),
25 μl ($169)
See all NDUFB4 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

15 kDa. ( Note )

Positive Control

HepG2

Predict Reactivity

Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human NDUFB4 Recombinant protein.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11167579

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

NADH:ubiquinone oxidoreductase subunit B4 , B15 , CI-B15

Cellular Localization

Mitochondrion inner membrane; Single-pass membrane protein; Matrix side

Background

The protein encoded by this gene belongs to the complex I NDUFB4 subunit family. Mammalian complex I is composed of 45 different subunits. This protein has NADH dehydrogenase activity and oxidoreductase activity. It transfers electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-NDUFB4 antibody used in IHC (Paraffin sections) (IHC-P). GTX111026

GTX111026 IHC-P Image

NDUFB4 antibody detects NDUFB4 protein at cytosol on human hepatoma by immunohistochemical analysis.
Sample: Paraffin-embedded hepatoma.
NDUFB4 antibody (GTX111026) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-NDUFB4 antibody used in Western Blot (WB). GTX111026

GTX111026 WB Image

Sample (30 ug of whole cell lysate)
A: HepG2
15% SDS PAGE
GTX111026 diluted at 1:1000

Anti-NDUFB4 antibody used in IHC (Paraffin sections) (IHC-P). GTX111026

GTX111026 IHC-P Image

Immunohistochemical analysis of paraffin-embedded RT2 xenograft, using NDUFB4(GTX111026) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-NDUFB4 antibody used in IHC (Paraffin sections) (IHC-P). GTX111026

GTX111026 IHC-P Image

Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using NDUFB4(GTX111026) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169