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NDUFB4 antibody

KO/KD
Anti-NDUFB4 antibody used in Western Blot (WB). GTX35219
Anti-NDUFB4 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX35219
Anti-NDUFB4 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX35219
Anti-NDUFB4 antibody used in Western Blot (WB). GTX35219

Cat. No. GTX35219

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human, Mouse
Package
100 μl ($399)
See all NDUFB4 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:100
Not tested in other applications.

Calculated MW

15 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-90 of human NDUFB4 (NP_001161803.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

NADH:ubiquinone oxidoreductase subunit B4 , B15 , CI-B15

Cellular Localization

Mitochondrion inner membrane

Background

This gene encodes a non-catalytic subunit of the multisubunit NADH:ubiquinone oxidoreductase, the first enzyme complex in the mitochondrial electron transport chain (complex I). Mammalian complex I is composed of 45 different subunits and transfers electrons from NADH to ubiquinone. [provided by RefSeq, Dec 2009]

Database

Research Area

DATA IMAGES

Anti-NDUFB4 antibody used in Western Blot (WB). GTX35219

GTX35219 WB Image

WB analysis of various sample lysates using GTX35219 NDUFB4 antibody.
Dilution : 1:3000
Loading : 25μg per lane

Anti-NDUFB4 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX35219

GTX35219 ICC/IF Image

ICC/IF analysis of L929 cells using GTX35219 NDUFB4 antibody.
Blue : DAPI
Dilution : 1:100

Anti-NDUFB4 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX35219

GTX35219 ICC/IF Image

ICC/IF analysis of U2OS cells using GTX35219 NDUFB4 antibody.
Blue : DAPI
Dilution : 1:100

Anti-NDUFB4 antibody used in Western Blot (WB). GTX35219

GTX35219 WB Image

KO/KD

WB analysis of normal (control) and knockout (KO) HeLa cell lysate using GTX35219 NDUFB4 antibody.
Dilution : 1:1000
Loading : 25μg per lane

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399