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NDUFB9 antibody

Anti-NDUFB9 antibody used in IHC (Paraffin sections) (IHC-P). GTX105590
Anti-NDUFB9 antibody used in Western Blot (WB). GTX105590
Anti-NDUFB9 antibody used in Western Blot (WB). GTX105590

Cat. No. GTX105590

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all NDUFB9 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

22 kDa. ( Note )

Positive Control

Mouse brain , HeLaS3 , HepG2

Predict Reactivity

Rat, Bovine, Xenopus tropicalis, Chimpanzee(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human NDUFB9. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950981

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

NADH:ubiquinone oxidoreductase subunit B9 , B22 , CI-B22 , LYRM3 , MC1DN24 , UQOR22

Cellular Localization

Mitochondrion inner membrane; Peripheral membrane protein; Matrix side

Background

Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed to be not involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.

Database

Research Area

DATA IMAGES

Anti-NDUFB9 antibody used in IHC (Paraffin sections) (IHC-P). GTX105590

GTX105590 IHC-P Image

NDUFB9 antibody detects NDUFB9 protein at cytosol on human gastric cancer by immunohistochemical analysis.
Sample: Paraffin-embedded gastric cancer.
NDUFB9 antibody (GTX105590) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-NDUFB9 antibody used in Western Blot (WB). GTX105590

GTX105590 WB Image

Sample(30 ug whole cell lysate)
A: HeLa S3(GTX14654)
B: Hep G2 (GTX27900)
12% SDS PAGE
GTX105590 diluted at 1:500

Anti-NDUFB9 antibody used in Western Blot (WB). GTX105590

GTX105590 WB Image

Sample (50 ug of whole cell lysate)
A: Mouse brain
12% SDS PAGE
GTX105590 diluted at 1:1000

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169