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NDUFB9 antibody

Anti-NDUFB9 antibody used in Western Blot (WB). GTX105590
Anti-NDUFB9 antibody used in IHC (Paraffin sections) (IHC-P). GTX105590
Anti-NDUFB9 antibody used in Western Blot (WB). GTX105590

Cat No. GTX105590

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human, Mouse
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

22 kDa. ( Note )

Positive Control

HeLaS3 , HepG2 , mouse brain

Predict Reactivity

Rat, Bovine, Chimpanzee(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human NDUFB9. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

NADH:ubiquinone oxidoreductase subunit B9 , B22 , CI-B22 , LYRM3 , MC1DN24 , UQOR22

Cellular Localization

Mitochondrion inner membrane; Peripheral membrane protein; Matrix side

Background

Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed to be not involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.

Database

Research Area

DATA IMAGES
Anti-NDUFB9 antibody used in Western Blot (WB). GTX105590

GTX105590 WB Image

Sample (50 ug of whole cell lysate)
A: Mouse brain
12% SDS PAGE
GTX105590 diluted at 1:1000

Anti-NDUFB9 antibody used in IHC (Paraffin sections) (IHC-P). GTX105590

GTX105590 IHC-P Image

NDUFB9 antibody detects NDUFB9 protein at cytosol on human gastric cancer by immunohistochemical analysis.
Sample: Paraffin-embedded gastric cancer.
NDUFB9 antibody (GTX105590) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-NDUFB9 antibody used in Western Blot (WB). GTX105590

GTX105590 WB Image

Sample(30 ug whole cell lysate)
A: HeLa S3(GTX14654)
B: Hep G2 (GTX27900)
12% SDS PAGE
GTX105590 diluted at 1:500

REFERENCE
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REVIEW
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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169