GeneTex
United States (US)

NDUFB9 antibody

GTX105590_39939_IHC_19011506_331.jpg
GTX105590_WB_19011506_227.jpg
GTX105590_39939_WB_M_brain_19011506_795.jpg

Cat No. GTX105590

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

22 kDa. ( Note )

Positive Control

HeLaS3 , HepG2 , mouse brain

Predict Reactivity

Rat, Bovine, Chimpanzee(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human NDUFB9. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

B22 antibody, DKFZp566O173 antibody, FLJ22885 antibody, LYRM3 antibody, UQOR22 antibody, NDUFB9 antibody, CI-B22 antibody, NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9 antibody, NADH-ubiquinone oxidoreductase B22 subunit antibody, complex I B22 subunit antibody, complex I-B22 antibody, LYR motif-containing protein 3 antibody, NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 9, 22kDa antibody

Cellular Localization

Mitochondrion inner membrane; Peripheral membrane protein; Matrix side

Background

Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed to be not involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.

Database

Research Area

DATA IMAGES
GTX105590_39939_IHC_19011506_331.jpg

GTX105590 IHC-P Image

NDUFB9 antibody detects NDUFB9 protein at cytosol on human gastric cancer by immunohistochemical analysis.
Sample: Paraffin-embedded gastric cancer.
NDUFB9 antibody (GTX105590) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX105590_WB_19011506_227.jpg

GTX105590 WB Image

Sample(30 ug whole cell lysate)
A: HeLa S3(GTX14654)
B: Hep G2 (GTX27900)
12% SDS PAGE
GTX105590 diluted at 1:500

GTX105590_39939_WB_M_brain_19011506_795.jpg

GTX105590 WB Image

Sample (50 ug of whole cell lysate)
A: Mouse brain
12% SDS PAGE
GTX105590 diluted at 1:1000

SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169