GeneTex
United States (US)

NDUFS1 antibody

GTX105270_39827_WB_R_brain_19011506_354.jpg
GTX105270_39827_IHC_19011506_113.jpg
GTX105270_WB_19011506_453.jpg
GTX105270_39827_WB_M_brain_19011506_475.jpg

Cat No. GTX105270

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:10000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

79 kDa. ( Note )

Positive Control

HepG2 , mouse brain , rat brain

Predict Reactivity

Bovine, Chicken, Xenopus laevis, Chimpanzee(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.2mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human NDUFS1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

CI-75Kd antibody, CI-75k antibody, MGC26839 antibody, PRO1304 antibody, NDUFS1 antibody, NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial antibody, mitochondrial NADH-ubiquinone oxidoreductase 75 kDa subunit antibody, complex I 75kDa subunit antibody, complex I, mitochondrial respiratory chain, 75-kD subunit antibody, NADH dehydrogenase (ubiquinone) Fe-S protein 1, 75kDa (NADH-coenzyme Q reductase) antibody

Cellular Localization

Mitochondrion inner membrane

Background

The protein encoded by this gene belongs to the complex I 75 kDa subunit family. Mammalian complex I is composed of 45 different subunits. It locates at the mitochondrial inner membrane. This protein has NADH dehydrogenase activity and oxidoreductase activity. It transfers electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. This protein is the largest subunit of complex I and it is a component of the iron-sulfur (IP) fragment of the enzyme. It may form part of the active site crevice where NADH is oxidized. Mutations in this gene are associated with complex I deficiency. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX105270_39827_WB_R_brain_19011506_354.jpg

GTX105270 WB Image

NDUFS1 antibody detects NDUFS1 protein by western blot analysis.
A. 50 µg rat brain lysate/extract
7.5% SDS-PAGE
NDUFS1 antibody (GTX105270) dilution: 1:500
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX105270_39827_IHC_19011506_113.jpg

GTX105270 IHC-P Image

NDUFS1 antibody detects NDUFS1 protein at mitochondria on human ovarian carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded ovarian carcinoma.
NDUFS1 antibody (GTX105270) dilution: 1:250.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX105270_WB_19011506_453.jpg

GTX105270 WB Image

Sample (30 µg of whole cell lysate)
A: HepG2 (GTX27900)
7.5% SDS PAGE
GTX105270 diluted at 1:1500
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX105270_39827_WB_M_brain_19011506_475.jpg

GTX105270 WB Image

Sample (50 µg of whole cell lysate)
A: Mouse brain
7.5% SDS PAGE
GTX105270 diluted at 1:10000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169