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NDUFS1 antibody

Orthogonal
Anti-NDUFS1 antibody used in Western Blot (WB). GTX113787
Anti-NDUFS1 antibody used in IHC (Paraffin sections) (IHC-P). GTX113787
Anti-NDUFS1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113787
Anti-NDUFS1 antibody used in Western Blot (WB). GTX113787

Cat. No. GTX113787

Reference 6

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all NDUFS1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

79 kDa. ( Note )

Predict Reactivity

Zebrafish, Bovine, Chicken, Chimpanzee(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.57 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human NDUFS1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2037498

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

NADH:ubiquinone oxidoreductase core subunit S1 , CI-75Kd , CI-75k , MC1DN5 , PRO1304

Cellular Localization

Mitochondrion inner membrane

Background

The protein encoded by this gene belongs to the complex I 75 kDa subunit family. Mammalian complex I is composed of 45 different subunits. It locates at the mitochondrial inner membrane. This protein has NADH dehydrogenase activity and oxidoreductase activity. It transfers electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. This protein is the largest subunit of complex I and it is a component of the iron-sulfur (IP) fragment of the enzyme. It may form part of the active site crevice where NADH is oxidized. Mutations in this gene are associated with complex I deficiency. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-NDUFS1 antibody used in Western Blot (WB). GTX113787

GTX113787 WB Image

Orthogonal

HepG2 and mitochondria extracts (30 μg) were separated by SDS-PAGE, and the membrane was blotted with NDUFS1 antibody (GTX113787) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-NDUFS1 antibody used in IHC (Paraffin sections) (IHC-P). GTX113787

GTX113787 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using NDUFS1(GTX113787) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-NDUFS1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113787

GTX113787 ICC/IF Image

Immunofluorescence analysis of methanol-fixed A431, using NDUFS1(GTX113787) antibody at 1:200 dilution.

Anti-NDUFS1 antibody used in Western Blot (WB). GTX113787

GTX113787 WB Image

Various tissue extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with NDUFS1 antibody (GTX113787) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Kuo TT et al. Molecules 2022; 27 (14) Quantitative Proteome Analysis Reveals Melissa officinalis Extract Targets Mitochondrial Respiration in Colon Cancer Cells.
Application : WB
Reactivity : Human
Lobo-Jarne T et al. EMBO J 2020; 39 (14) : e103912 Multiple pathways coordinate assembly of human mitochondrial complex IV and stabilization of respiratory supercomplexes.
Application : WB
Reactivity : Human
Fujimaki M et al. Mol Cell Biol 2019; (Epub) Iron supply via NCOA4-mediated ferritin degradation maintains mitochondrial functions.
Application : WB
Reactivity : Human
Ishiyama A et al. Neurol Genet 2017; 3 (5) : e184 IBA57 mutations abrogate iron-sulfur cluster assembly leading to cavitating leukoencephalopathy.
Application : WB
Reactivity : Human
Perez-Perez R et al. Cell Rep 2016; 16 (9) : 2387-98 COX7A2L Is a Mitochondrial Complex III Binding Protein that Stabilizes the III2+IV Supercomplex without Affecting Respirasome Formation.
Application : WB
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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169