NDUFS2 antibody

Anti-NDUFS2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX04733

Cat. No. GTX04733

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, IHC-P, FACS, ELISA
Reactivity	Human, Mouse, Rat
Package	100 μg ($469)

See all NDUFS2 products

Datasheet

View product citations for antibody GTX04733 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	0.1-0.25 μg/mL
ICC/IF	5 μg/mL
IHC-P	2-5 μg/mL
FACS	1-3 μg/10⁶ cells
ELISA	0.1-0.5 μg/mL

Not tested in other applications.

Calculated MW

53 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na₂HPO₄

Preservative

No preservatives

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.5 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

E.coli-derived human NDUFS2 recombinant protein (Position: M1-R463).

Purity

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

CI49,NADH:ubiquinone oxidoreductase core subunit S2,NDUFS2

Cellular Localization

Mitochondrion inner membrane

Background

The protein encoded by this gene is a core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (complex I). Mammalian mitochondrial complex I is composed of at least 43 different subunits, 7 of which are encoded by the mitochondrial genome, and the rest are the products of nuclear genes. The iron-sulfur protein fraction of complex I is made up of 7 subunits, including this gene product. Complex I catalyzes the NADH oxidation with concomitant ubiquinone reduction and proton ejection out of the mitochondria. Mutations in this gene are associated with mitochondrial complex I deficiency. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Oct 2009]

Database

Gene ID: 4720 NDUFS2
UniProt: O75306 NDUFS2

Research Area

DATA IMAGES

GTX04733 IHC-P Image

IHC-P analysis of mouse heart tissue using GTX04733 NDUFS2 antibody.
Antigen retrieval : Heat mediated EDTA buffer (pH 8.0)
Dilution : 2 μg/mL

GTX04733 IHC-P Image

IHC-P analysis of human liver cancer tissue using GTX04733 NDUFS2 antibody.
Red : Primary antibody
Blue : DAPI
Antigen retrieval : Heat mediated EDTA buffer (pH 8.0)
Dilution : 5 μg/mL

GTX04733 IHC-P Image

IHC-P analysis of human breast cancer tissue using GTX04733 NDUFS2 antibody.
Antigen retrieval : Heat mediated EDTA buffer (pH 8.0)
Dilution : 2 μg/mL

GTX04733 IHC-P Image

IHC-P analysis of human liver cancer tissue using GTX04733 NDUFS2 antibody.
Antigen retrieval : Heat mediated EDTA buffer (pH 8.0)
Dilution : 2 μg/mL

GTX04733 IHC-P Image

IHC-P analysis of human lung cancer tissue using GTX04733 NDUFS2 antibody.
Antigen retrieval : Heat mediated EDTA buffer (pH 8.0)
Dilution : 2 μg/mL

GTX04733 IHC-P Image

IHC-P analysis of human colorectal adenocarcinoma tissue using GTX04733 NDUFS2 antibody.
Antigen retrieval : Heat mediated EDTA buffer (pH 8.0)
Dilution : 2 μg/mL

GTX04733 IHC-P Image

IHC-P analysis of human endometrial adenocarcinoma tissue using GTX04733 NDUFS2 antibodyy.
Antigen retrieval : Heat mediated EDTA buffer (pH 8.0)
Dilution : 2 μg/mL

GTX04733 IHC-P Image

IHC-P analysis of rat cardiac muscle tissue using GTX04733 NDUFS2 antibody.
Red : Primary antibody
Blue : DAPI
Antigen retrieval : Heat mediated EDTA buffer (pH 8.0)
Dilution : 5 μg/mL

GTX04733 FACS Image

FACS analysis of HeLa cells using GTX04733 NDUFS2 antibody.
Blue : Primary antibody
Green : Isotype control
Red : Unlabeled samples
Dilution : 1 μg/10⁶ cells

GTX04733 WB Image

WB analysis of various samples using GTX04733 NDUFS2 antibody.
Lane 1 : HeLa whole cell lysate
Lane 2 : Jurkat whole cell lysate
Lane 3 : 293T whole cell lysate
Lane 4 : CCRF-CEM whole cell lysate
Lane 5 : Rat brain tissue lysate
Lane 6 : Rat lung tissue lysate
Lane 7 : Mouse brain tissue lysate
Lane 8 : Mouse lung tissue lysate
Loading : 30 μg
Dilution : 0.25 μg/mL

GTX04733 ICC/IF Image

ICC/IF analysis of PFA fixed CaCo2 cells using GTX04733 NDUFS2 antibody.
Antigen retrieval : IHC enzyme antigen retrieval reagent
Green : Primary antibody
Blue : DAPI
Dilution : 5 μg/mL

REFERENCE

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REVIEW

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Package

List Price ($)

100 μg

$ 469

Qty

NDUFS2 antibody

Cat. No. GTX04733

Host

Clonality

Isotype

Application

Reactivity

APPLICATION

Application Note

Calculated MW

PROPERTIES

Form

Buffer

Preservative

Storage

Concentration

Antigen Species

Immunogen

Purity

Conjugation

Note

TARGET

Synonyms

Cellular Localization

Background

Database

Research Area

DATA IMAGES

GTX04733 IHC-P Image

GTX04733 IHC-P Image

GTX04733 IHC-P Image

GTX04733 IHC-P Image

GTX04733 IHC-P Image

GTX04733 IHC-P Image

GTX04733 IHC-P Image

GTX04733 IHC-P Image

GTX04733 FACS Image

GTX04733 WB Image

GTX04733 ICC/IF Image

REFERENCE

REVIEW

Product

Research

Support

Company

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