GeneTex
United States (US)

NDUFS2 antibody

GTX114924_40240_20160421_WB_R_brain_18121410_848.jpg
GTX114924_40240_IHC_18121410_929.jpg
GTX114924_WB_18121410_913.jpg
GTX114924_40240_WB_M_brain_18121410_255.jpg
GTX114924_40240_IFA_18121410_621.jpg

Cat No. GTX114924

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

53 kDa. ( Note )

Positive Control

293T , A431 , Jurkat , Raji , mouse brain , rat brain

Predict Reactivity

Zebrafish, Bovine, Xenopus laevis, Chimpanzee, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.89 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human NDUFS2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Nadh:Ubiquinone Oxidoreductase Core Subunit S2,Ci-49,Ndufs2

Cellular Localization

Mitochondrion inner membrane; Peripheral membrane protein; Matrix side

Background

Mitochondrial complex I (NADH-ubiquinone reductase; EC 1.6.5.3) is the first multimeric complex of the respiratory chain that catalyzes the NADH oxidation with concomitant ubiquinone reduction and proton ejection out of the mitochondria. Mammalian mitochondrial complex I is an assembly of at least 43 different subunits. Seven of the subunits are encoded by the mitochondrial genome; the remainder are the products of nuclear genes. The iron-sulfur protein (IP) fraction of complex I is made up of 7 subunits, including NDUFS2. See NDUFS1 (MIM 157655).[supplied by OMIM]

Database

Research Area

DATA IMAGES
GTX114924_40240_20160421_WB_R_brain_18121410_848.jpg

GTX114924 WB Image

Rat tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with NDUFS2 antibody (GTX114924) diluted at 1:1000.

GTX114924_40240_IHC_18121410_929.jpg

GTX114924 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using NDUFS2(GTX114924) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX114924_WB_18121410_913.jpg

GTX114924 WB Image

Sample (30 ug of whole cell lysate)
A: 293T
B: A431 (GTX27909)
C: JurKat
D: Raji
10% SDS PAGE
GTX114924 diluted at 1:1000

GTX114924_40240_WB_M_brain_18121410_255.jpg

GTX114924 WB Image

Sample (50 ug of whole cell lysate)
A: Mouse brain
10% SDS PAGE
GTX114924 diluted at 1:10000

GTX114924_40240_IFA_18121410_621.jpg

GTX114924 ICC/IF Image

NDUFS2 antibody detects NDUFS2 protein at mitochondria by immunofluorescent analysis.
Sample: HeLa cells were fixed in 2% paraformaldehyde/culture medium at 37℃ for 30 min.
Green: NDUFS2 protein stained by NDUFS2 antibody (GTX114924) diluted at 1:500.
Blue: Hoechst 33343 staining.

REFERENCE
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169