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NDUFS2 antibody

Anti-NDUFS2 antibody used in Western Blot (WB). GTX114924
Anti-NDUFS2 antibody used in Western Blot (WB). GTX114924
Anti-NDUFS2 antibody used in IHC (Paraffin sections) (IHC-P). GTX114924
Anti-NDUFS2 antibody used in Western Blot (WB). GTX114924
Anti-NDUFS2 antibody used in Western Blot (WB). GTX114924

Cat. No. GTX114924

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P, IP

Reactivity

Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:10000
IHC-P 1:100-1:1000
IP Assay dependent
Not tested in other applications.

Calculated MW

53 kDa. ( Note )

Positive Control

Mouse brain , 293T , A431 , Jurkat , Raji , rat brain

Predict Reactivity

Zebrafish, Bovine, Xenopus tropicalis, Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.43 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human NDUFS2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10624594

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

NADH:ubiquinone oxidoreductase core subunit S2 , CI-49 , MC1DN6

Cellular Localization

Mitochondrion inner membrane; Peripheral membrane protein; Matrix side

Background

Mitochondrial complex I (NADH-ubiquinone reductase; EC 1.6.5.3) is the first multimeric complex of the respiratory chain that catalyzes the NADH oxidation with concomitant ubiquinone reduction and proton ejection out of the mitochondria. Mammalian mitochondrial complex I is an assembly of at least 43 different subunits. Seven of the subunits are encoded by the mitochondrial genome; the remainder are the products of nuclear genes. The iron-sulfur protein (IP) fraction of complex I is made up of 7 subunits, including NDUFS2. See NDUFS1 (MIM 157655).[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-NDUFS2 antibody used in Western Blot (WB). GTX114924

GTX114924 WB Image

Sample (30 ug of whole cell lysate)
A: 293T
B: A431 (GTX27909)
C: JurKat
D: Raji
10% SDS PAGE
GTX114924 diluted at 1:1000

Anti-NDUFS2 antibody used in Western Blot (WB). GTX114924

GTX114924 WB Image

Sample (50 ug of whole cell lysate)
A: Mouse brain
10% SDS PAGE
GTX114924 diluted at 1:10000

Anti-NDUFS2 antibody used in IHC (Paraffin sections) (IHC-P). GTX114924

GTX114924 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using NDUFS2(GTX114924) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-NDUFS2 antibody used in Western Blot (WB). GTX114924

GTX114924 WB Image

Rat tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with NDUFS2 antibody (GTX114924) diluted at 1:1000.

Anti-NDUFS2 antibody used in Western Blot (WB). GTX114924

GTX114924 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with NDUFS2 antibody (GTX114924) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

REVIEW

NDUFS2 antibody Cat. No. GTX114924

Rating ( Average 5 based on 2 users reviews)
Application
Western Blot(WB) ( Average 5 based on 1 users reviews)
Immunoprecipitation(IP) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 08-Jun-2022
Score :
Application Tested : IP
Sample Species : Hu
Sample : HAP1 cells
Primary Antibody : 1:1000, 4°C, > 12Hr
Lane Description :
SM: 10% starting material
UB: 10% unbound fraction
IP: IP antibody
Figure Note : Antibody-bead conjugates were prepared by adding 2.0 μg of antibody to 500 μl of IP Lysis Buffer, together with 30μl of protein A-Sepharose beads. One ml aliquots at 0.5 mg/ml of lysate were incubated with an antibody-bead conjugate for ~2 hrs at 4ºC.
Data provided by YCharOS Inc., an open science company with the mission of characterizing commercially available antibody reagents for all human proteins using knockout technology.
Date : Anonymous submitted on 08-Jun-2022
Score :
Application Tested : WB
Sample Species : Hu
Sample : HAP1 WT and NDUFS2 KO cells
Amount used : 40μg
Primary Antibody : 1:1000, 4°C, > 12Hr
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169