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NDUFS2 antibody

KO/KD
Anti-NDUFS2 antibody used in Western Blot (WB). GTX35213
Anti-NDUFS2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX35213
Anti-NDUFS2 antibody used in Western Blot (WB). GTX35213

Cat. No. GTX35213

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399)
See all NDUFS2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000 - 1:3000
ICC/IF 1:50 - 1:200
Not tested in other applications.

Calculated MW

53 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 204-463 of human NDUFS2 (NP_004541.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

NADH:ubiquinone oxidoreductase core subunit S2 , CI-49 , MC1DN6

Cellular Localization

Mitochondrion inner membrane

Background

The protein encoded by this gene is a core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (complex I). Mammalian mitochondrial complex I is composed of at least 43 different subunits, 7 of which are encoded by the mitochondrial genome, and the rest are the products of nuclear genes. The iron-sulfur protein fraction of complex I is made up of 7 subunits, including this gene product. Complex I catalyzes the NADH oxidation with concomitant ubiquinone reduction and proton ejection out of the mitochondria. Mutations in this gene are associated with mitochondrial complex I deficiency. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Oct 2009]

Database

Research Area

DATA IMAGES

Anti-NDUFS2 antibody used in Western Blot (WB). GTX35213

GTX35213 WB Image

WB analysis of various sample lysates using GTX35213 NDUFS2 antibody.
Dilution : 1:3000
Loading : 25μg per lane

Anti-NDUFS2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX35213

GTX35213 ICC/IF Image

ICC/IF analysis of L929 cells using GTX35213 NDUFS2 antibody.
Blue : DAPI
Dilution : 1:100

Anti-NDUFS2 antibody used in Western Blot (WB). GTX35213

GTX35213 WB Image

KO/KD

WB analysis of normal (control) and knockout (KO) 293T cell lysate using GTX35213 NDUFS2 antibody.
Dilution : 1:3000
Loading : 25μg per lane

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399