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NDUFS3 antibody [HL2097]

Recombinant antibody KO/KD
Anti-NDUFS3 antibody [HL2097] used in Western Blot (WB). GTX638005
Anti-NDUFS3 antibody [HL2097] used in IHC (Paraffin sections) (IHC-P). GTX638005
Anti-NDUFS3 antibody [HL2097] used in IHC (Paraffin sections) (IHC-P). GTX638005
Anti-NDUFS3 antibody [HL2097] used in Western Blot (WB). GTX638005
Anti-NDUFS3 antibody [HL2097] used in Western Blot (WB). GTX638005
Anti-NDUFS3 antibody [HL2097] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX638005
Anti-NDUFS3 antibody [HL2097] used in Western Blot (WB). GTX638005

Cat. No. GTX638005

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 HL2097

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat, Zebrafish
Package
100 μl ($399),
25 μl ($169)
See all NDUFS3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

30 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.5 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fragment of human NDUFS3

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

NADH:ubiquinone oxidoreductase core subunit S3 , CI-30 , MC1DN8

Cellular Localization

Mitochondrion inner membrane

Background

This gene encodes one of the iron-sulfur protein (IP) components of mitochondrial NADH:ubiquinone oxidoreductase (complex I). Mutations in this gene are associated with Leigh syndrome resulting from mitochondrial complex I deficiency.[provided by RefSeq, Apr 2009]

Database

Research Area

DATA IMAGES

Anti-NDUFS3 antibody [HL2097] used in Western Blot (WB). GTX638005

GTX638005 WB Image

KO/KD

Wild-type (WT) and NDUFS3 knockout (KO) 293T cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with NDUFS3 antibody [HL2097] (GTX638005) diluted at 1:2500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-NDUFS3 antibody [HL2097] used in IHC (Paraffin sections) (IHC-P). GTX638005

GTX638005 IHC-P Image

NDUFS3 antibody [HL2097] detects NDUFS3 protein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse tissues.
NDUFS3 stained by NDUFS3 antibody [HL2097] (GTX638005) diluted at 1:50.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-NDUFS3 antibody [HL2097] used in IHC (Paraffin sections) (IHC-P). GTX638005

GTX638005 IHC-P Image

NDUFS3 antibody [HL2097] detects NDUFS3 protein by immunohistochemical analysis.
Sample: Paraffin-embedded rat tissues.
NDUFS3 stained by NDUFS3 antibody [HL2097] (GTX638005) diluted at 1:50.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-NDUFS3 antibody [HL2097] used in Western Blot (WB). GTX638005

GTX638005 WB Image

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with NDUFS3 antibody [HL2097] (GTX638005) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-NDUFS3 antibody [HL2097] used in Western Blot (WB). GTX638005

GTX638005 WB Image

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with NDUFS3 antibody [HL2097] (GTX638005) diluted at 1:2500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-NDUFS3 antibody [HL2097] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX638005

GTX638005 ICC/IF Image

NDUFS3 antibody [HL2097] detects NDUFS3 protein at mitochondria by immunofluorescent analysis.
Sample: 293T cells were fixed in ice-cold MeOH for 5 min.
Green: NDUFS3 stained by NDUFS3 antibody [HL2097] (GTX638005) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-NDUFS3 antibody [HL2097] used in Western Blot (WB). GTX638005

GTX638005 WB Image

Whole zebrafish extract (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with NDUFS3 antibody [HL2097] (GTX638005) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

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Package List Price ($)
$ 399
$ 169
Custom Conjugations