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NDUFS3 antibody

KO/KD
Anti-NDUFS3 antibody used in Western Blot (WB). GTX113504
Anti-NDUFS3 antibody used in Western Blot (WB). GTX113504
Anti-NDUFS3 antibody used in IHC (Paraffin sections) (IHC-P). GTX113504
Anti-NDUFS3 antibody used in Western Blot (WB). GTX113504

Cat. No. GTX113504

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all NDUFS3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

30 kDa. ( Note )

Positive Control

THP-1 , HL-60 , Jurkat , NCI-H929 , 293T

Predict Reactivity

Mouse, Rat, Zebrafish, Bovine, Chicken, Pig, Xenopus tropicalis, Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.54 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human NDUFS3. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11172615

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

NADH:ubiquinone oxidoreductase core subunit S3 , CI-30 , MC1DN8

Cellular Localization

Mitochondrion inner membrane

Background

This gene encodes one of the iron-sulfur protein (IP) components of mitochondrial NADH:ubiquinone oxidoreductase (complex I). Mutations in this gene are associated with Leigh syndrome resulting from mitochondrial complex I deficiency.

Database

Research Area

DATA IMAGES

Anti-NDUFS3 antibody used in Western Blot (WB). GTX113504

GTX113504 WB Image

KO/KD

Wild-type (WT) and NDUFS3 knockout (KO) 293T cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with NDUFS3 antibody (GTX113504) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-NDUFS3 antibody used in Western Blot (WB). GTX113504

GTX113504 WB Image

NDUFS3 antibody detects NDUFS3 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with NDUFS3 antibody (GTX113504) diluted by 1:1000.

Anti-NDUFS3 antibody used in IHC (Paraffin sections) (IHC-P). GTX113504

GTX113504 IHC-P Image

Immunohistochemical analysis of paraffin-embedded U87 xenograft, using NDUFS3(GTX113504) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-NDUFS3 antibody used in Western Blot (WB). GTX113504

GTX113504 WB Image

Sample (30 ug of whole cell lysate)
A: THP-1
B: HL-60
12% SDS PAGE
GTX113504 diluted at 1:1000

REFERENCE

Application Reference

Boominathan A et al. Nucleic Acids Res 2016; Stable nuclear expression of ATP8 and ATP6 genes rescues a mtDNA Complex V null mutant.
Application : WB
Lim SC et al. FASEB J 2016; Loss of mitochondrial DNA-encoded protein ND1 results in disruption of complex I biogenesis during early stages of assembly.
Application : WB
Reactivity : Human
Submit a Reference

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169