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NDUFS4 antibody

Anti-NDUFS4 antibody used in Western Blot (WB). GTX105662
Anti-NDUFS4 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX105662
Anti-NDUFS4 antibody used in Western Blot (WB). GTX105662
Anti-NDUFS4 antibody used in Western Blot (WB). GTX105662
Anti-NDUFS4 antibody used in IHC (Paraffin sections) (IHC-P). GTX105662

Cat. No. GTX105662

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all NDUFS4 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

20 kDa. ( Note )

Positive Control

Mouse brain , Rat heart , 293T

Predict Reactivity

Zebrafish, Bovine, Chimpanzee(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human NDUFS4. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950983

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

NADH:ubiquinone oxidoreductase subunit S4 , AQDQ , CI-18 , CI-18 kDa , CI-AQDQ , MC1DN1

Cellular Localization

Mitochondrion inner membrane; Peripheral membrane protein; Matrix side

Background

This gene encodes an accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), or NADH:ubiquinone oxidoreductase, the first multi-subunit enzyme complex of the mitochondrial respiratory chain. Complex I plays a vital role in cellular ATP production, the primary source of energy for many crucial processes in living cells. It removes electrons from NADH and passes them by a series of different protein-coupled redox centers to the electron acceptor ubiquinone. In well-coupled mitochondria, the electron flux leads to ATP generation via the building of a proton gradient across the inner membrane. Complex I is composed of at least 41 subunits, of which 7 are encoded by the mitochondrial genome and the remainder by nuclear genes. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-NDUFS4 antibody used in Western Blot (WB). GTX105662

GTX105662 WB Image

Sample(30 μg whole cell lysate)
A: 293T
12% SDS PAGE
GTX105662 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-NDUFS4 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX105662

GTX105662 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using NDUFS4(GTX105662) antibody at 1:100 dilution.

Anti-NDUFS4 antibody used in Western Blot (WB). GTX105662

GTX105662 WB Image

NDUFS4 antibody detects NDUFS4 protein by western blot analysis.
A. 50 μg Rat heart lysate/extract
12% SDS-PAGE
NDUFS4 antibody (GTX105662) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-NDUFS4 antibody used in Western Blot (WB). GTX105662

GTX105662 WB Image

Sample (50 μg of whole cell lysate)
A: Mouse brain
12% SDS PAGE
GTX105662 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-NDUFS4 antibody used in IHC (Paraffin sections) (IHC-P). GTX105662

GTX105662 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human ovarian cancer, using NDUFS4(GTX105662) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

Application Reference

Lim SC et al. FASEB J 2016; Loss of mitochondrial DNA-encoded protein ND1 results in disruption of complex I biogenesis during early stages of assembly.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169