GeneTex
United States (US)

NDUFS4 antibody

GTX105662_39946_WB_M_brain_19011506_598.jpg
GTX105662_WB_19011506_209.jpg
GTX105662_39946_WB_R_heart_19011506_995.jpg
GTX105662_IHC_19011506_550.jpg
GTX105662_39946_IFA_19011506_617.jpg

Cat No. GTX105662

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

20 kDa. ( Note )

Positive Control

293T , mouse brain , rat heart

Predict Reactivity

Zebrafish, Bovine, Chimpanzee(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human NDUFS4. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

AQDQ antibody, CI-18 antibody, NDUFS4 antibody, CI-AQDQ antibody, CI-18 kDa antibody, "NADH-coenzyme Q reductase, 18-KD antibody", mitochondrial respiratory chain complex I (18-KD subunit) antibody, complex I 18kDa subunit antibody, complex I-AQDQ antibody, NADH dehydrogenase (ubiquinone) iron-sulfur protein 4 antibody, complex I-18 kDa antibody, "NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial antibody", NADH-ubiquinone oxidoreductase 18 kDa subunit antibody, "NADH dehydrogenase (ubiquinone) Fe-S protein 4, 18kDa (NADH-coenzyme Q reductase) antibody"

Cellular Localization

Mitochondrion inner membrane; Peripheral membrane protein; Matrix side

Background

This gene encodes an accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), or NADH:ubiquinone oxidoreductase, the first multi-subunit enzyme complex of the mitochondrial respiratory chain. Complex I plays a vital role in cellular ATP production, the primary source of energy for many crucial processes in living cells. It removes electrons from NADH and passes them by a series of different protein-coupled redox centers to the electron acceptor ubiquinone. In well-coupled mitochondria, the electron flux leads to ATP generation via the building of a proton gradient across the inner membrane. Complex I is composed of at least 41 subunits, of which 7 are encoded by the mitochondrial genome and the remainder by nuclear genes. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX105662_39946_WB_M_brain_19011506_598.jpg

GTX105662 WB Image

Sample (50 µg of whole cell lysate)
A: Mouse brain
12% SDS PAGE
GTX105662 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX105662_WB_19011506_209.jpg

GTX105662 WB Image

Sample(30 µg whole cell lysate)
A: 293T
12% SDS PAGE
GTX105662 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX105662_39946_WB_R_heart_19011506_995.jpg

GTX105662 WB Image

NDUFS4 antibody detects NDUFS4 protein by western blot analysis.
A. 50 µg Rat heart lysate/extract
12% SDS-PAGE
NDUFS4 antibody (GTX105662) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX105662_IHC_19011506_550.jpg

GTX105662 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human ovarian cancer, using NDUFS4(GTX105662) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX105662_39946_IFA_19011506_617.jpg

GTX105662 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using NDUFS4(GTX105662) antibody at 1:100 dilution.

REFERENCE
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169