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NDUFS4 antibody

Anti-NDUFS4 antibody used in Western Blot (WB). GTX105662
Anti-NDUFS4 antibody used in Western Blot (WB). GTX105662
Anti-NDUFS4 antibody used in Western Blot (WB). GTX105662
Anti-NDUFS4 antibody used in IHC (Paraffin sections) (IHC-P). GTX105662
Anti-NDUFS4 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX105662

Cat No. GTX105662

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse, Rat
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

20 kDa. ( Note )

Positive Control

293T , mouse brain , rat heart

Predict Reactivity

Zebrafish, Bovine, Chimpanzee(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human NDUFS4. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

NADH:ubiquinone oxidoreductase subunit S4 , AQDQ , CI-18 , CI-18 kDa , CI-AQDQ , MC1DN1

Cellular Localization

Mitochondrion inner membrane; Peripheral membrane protein; Matrix side

Background

This gene encodes an accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), or NADH:ubiquinone oxidoreductase, the first multi-subunit enzyme complex of the mitochondrial respiratory chain. Complex I plays a vital role in cellular ATP production, the primary source of energy for many crucial processes in living cells. It removes electrons from NADH and passes them by a series of different protein-coupled redox centers to the electron acceptor ubiquinone. In well-coupled mitochondria, the electron flux leads to ATP generation via the building of a proton gradient across the inner membrane. Complex I is composed of at least 41 subunits, of which 7 are encoded by the mitochondrial genome and the remainder by nuclear genes. [provided by RefSeq]

Database

Research Area

DATA IMAGES
Anti-NDUFS4 antibody used in Western Blot (WB). GTX105662

GTX105662 WB Image

Sample (50 µg of whole cell lysate)
A: Mouse brain
12% SDS PAGE
GTX105662 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-NDUFS4 antibody used in Western Blot (WB). GTX105662

GTX105662 WB Image

Sample(30 µg whole cell lysate)
A: 293T
12% SDS PAGE
GTX105662 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-NDUFS4 antibody used in Western Blot (WB). GTX105662

GTX105662 WB Image

NDUFS4 antibody detects NDUFS4 protein by western blot analysis.
A. 50 µg Rat heart lysate/extract
12% SDS-PAGE
NDUFS4 antibody (GTX105662) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-NDUFS4 antibody used in IHC (Paraffin sections) (IHC-P). GTX105662

GTX105662 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human ovarian cancer, using NDUFS4(GTX105662) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-NDUFS4 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX105662

GTX105662 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using NDUFS4(GTX105662) antibody at 1:100 dilution.

REFERENCE
REVIEW
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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169