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NDUFS5 antibody

Anti-NDUFS5 antibody used in Western Blot (WB). GTX101829
Anti-NDUFS5 antibody used in IHC (Paraffin sections) (IHC-P). GTX101829
Anti-NDUFS5 antibody used in IHC (Paraffin sections) (IHC-P). GTX101829

Cat. No. GTX101829

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Rat
Package
100 μl ($419),
25 μl ($169)
See all NDUFS5 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

13 kDa. ( Note )

Positive Control

Raji

Predict Reactivity

Pig, Chimpanzee(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human NDUFS5. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1241096

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

NADH:ubiquinone oxidoreductase subunit S5 , CI-15k , CI15K

Cellular Localization

Mitochondrion inner membrane; Peripheral membrane protein; Matrix side

Background

This gene is a member of the NADH dehydrogenase (ubiquinone) iron-sulfur protein family. The encoded protein is a subunit of the NADH:ubiquinone oxidoreductase (complex I), the first enzyme complex in the electron transport chain located in the inner mitochondrial membrane. Alternative splicing results in multiple transcript variants and pseudogenes have been identified on chromosomes 1, 4 and 17. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-NDUFS5 antibody used in Western Blot (WB). GTX101829

GTX101829 WB Image

Sample (30 ug of whole cell lysate)
A: Raji
15% SDS PAGE
GTX101829 diluted at 1:1000

Anti-NDUFS5 antibody used in IHC (Paraffin sections) (IHC-P). GTX101829

GTX101829 IHC-P Image

NDUFS5 antibody detects NDUFS5 protein at mitochondria on human lung adenocarcinoma by immunohistochemical analysis.
Sample: NDUFS5 antibody (GTX101829) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-NDUFS5 antibody used in IHC (Paraffin sections) (IHC-P). GTX101829

GTX101829 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human breast cancer, using NDUFS5(GTX101829) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

Application Reference

Lee S et al. Cell Res 2011; 21 (5) : 817-34 Mitochondrial H2O2 generated from electron transport chain complex I stimulates muscle differentiation.
Application : WB
Reactivity : Rat
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 419
$ 169