GeneTex
United States (US)

NDUFS5 antibody

GTX101829_40302_WB_19011506_871.jpg
GTX101829_40302_IHC_2_19011506_906.jpg
GTX101829_40302_IHC_19011506_593.jpg

Cat No. GTX101829

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

13 kDa. ( Note )

Positive Control

293T , Raji

Predict Reactivity

Pig, Chimpanzee(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human NDUFS5. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Nadh:Ubiquinone Oxidoreductase Subunit S5,Ci-15K,Ci15K,Ndufs5

Cellular Localization

Mitochondrion inner membrane; Peripheral membrane protein; Matrix side

Background

This gene is a member of the NADH dehydrogenase (ubiquinone) iron-sulfur protein family. The encoded protein is a subunit of the NADH:ubiquinone oxidoreductase (complex I), the first enzyme complex in the electron transport chain located in the inner mitochondrial membrane. Alternative splicing results in multiple transcript variants and pseudogenes have been identified on chromosomes 1, 4 and 17. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX101829_40302_WB_19011506_871.jpg

GTX101829 WB Image

Sample (30 ug of whole cell lysate)
A: Raji
15% SDS PAGE
GTX101829 diluted at 1:1000

GTX101829_40302_IHC_2_19011506_906.jpg

GTX101829 IHC-P Image

NDUFS5 antibody detects NDUFS5 protein at mitochondria on human lung adenocarcinoma by immunohistochemical analysis.
Sample: NDUFS5 antibody (GTX101829) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX101829_40302_IHC_19011506_593.jpg

GTX101829 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human breast cancer, using NDUFS5(GTX101829) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169