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NDUFS8 antibody

Anti-NDUFS8 antibody used in IHC (Paraffin sections) (IHC-P). GTX114119
Anti-NDUFS8 antibody used in Western Blot (WB). GTX114119
Anti-NDUFS8 antibody used in IHC (Paraffin sections) (IHC-P). GTX114119
Anti-NDUFS8 antibody used in IHC (Paraffin sections) (IHC-P). GTX114119
Anti-NDUFS8 antibody used in Western Blot (WB). GTX114119
Anti-NDUFS8 antibody used in Western Blot (WB). GTX114119

Cat. No. GTX114119

Reference 3

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($419),
25 μl ($169)
See all NDUFS8 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

24 kDa. ( Note )

Positive Control

Mouse brain , rat brain , HepG2

Predict Reactivity

Bovine, Dog, Xenopus tropicalis, Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.35 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human NDUFS8. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10619889

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

NADH:ubiquinone oxidoreductase core subunit S8 , CI-23k , CI23KD , MC1DN2 , TYKY

Cellular Localization

Mitochondrion

Background

This gene encodes a subunit of mitochondrial NADH:ubiquinone oxidoreductase, or Complex I, a multimeric enzyme of the respiratory chain responsible for NADH oxidation, ubiquinone reduction, and the ejection of protons from mitochondria. The encoded protein is involved in the binding of two of the six to eight iron-sulfur clusters of Complex I and, as such, is required in the electron transfer process. Mutations in this gene have been associated with Leigh syndrome. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-NDUFS8 antibody used in IHC (Paraffin sections) (IHC-P). GTX114119

GTX114119 IHC-P Image

NDUFS8 antibody detects NDUFS8 protein at on mouse stomach by immunohistochemical analysis.
Sample: Paraffin-embedded mouse stomach.
NDUFS8 antibody (GTX114119) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-NDUFS8 antibody used in Western Blot (WB). GTX114119

GTX114119 WB Image

Sample (30 ug of whole cell lysate)
A: Hep G2 (GTX27900)
12% SDS PAGE
NDUFS8 antibody
GTX114119 diluted at 1:1000

Anti-NDUFS8 antibody used in IHC (Paraffin sections) (IHC-P). GTX114119

GTX114119 IHC-P Image

NDUFS8 antibody detects NDUFS8 protein at cytosol on U87 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded U87 xenograft.
NDUFS8 antibody (GTX114119) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-NDUFS8 antibody used in IHC (Paraffin sections) (IHC-P). GTX114119

GTX114119 IHC-P Image

NDUFS8 antibody detects NDUFS8 protein at on mouse intestine by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
NDUFS8 antibody (GTX114119) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-NDUFS8 antibody used in Western Blot (WB). GTX114119

GTX114119 WB Image

NDUFS8 antibody detects NDUFS8 protein by Western blot analysis.
A. 50 μg rat brain lysate/extract
12 % SDS-PAGE
NDUFS8 antibody (GTX114119) dilution: 1:1000

Anti-NDUFS8 antibody used in Western Blot (WB). GTX114119

GTX114119 WB Image

Sample (50 ug of whole cell lysate)
A: mouse brain
12% SDS PAGE
GTX114119 diluted at 1:1000

REFERENCE

Application Reference

Paula Garcia-Esparcia et al. Brain Pathol 2018; 28 (1) : 43-57 Mitochondrial activity in the frontal cortex area 8 and angular gyrus in Parkinson's disease and Parkinson's disease with dementia.
Application : WB
Reactivity : Human
Ishiyama A et al. Neurol Genet 2017; 3 (5) : e184 IBA57 mutations abrogate iron-sulfur cluster assembly leading to cavitating leukoencephalopathy.
Application : WB
Reactivity : Human
Lim SC et al. FASEB J 2016; Loss of mitochondrial DNA-encoded protein ND1 results in disruption of complex I biogenesis during early stages of assembly.
Application : WB
Reactivity : Human
Submit a Reference

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 419
$ 169