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NDUFV1 antibody [HL1600]

Recombinant antibody KO/KD
Anti-NDUFV1 antibody [HL1600] used in Western Blot (WB). GTX637079
Anti-NDUFV1 antibody [HL1600] used in IHC (Paraffin sections) (IHC-P). GTX637079
Anti-NDUFV1 antibody [HL1600] used in Western Blot (WB). GTX637079

Cat. No. GTX637079

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 HL1600

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Rat
Package
100 μl ($399),
25 μl ($169)
See all NDUFV1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

51 kDa. ( Note )

Predict Reactivity

Mouse, Zebrafish, Bovine, Chicken, Xenopus tropicalis, Chimpanzee(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human NDUFV1. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

NADH:ubiquinone oxidoreductase core subunit V1 , CI-51K , CI51KD , MC1DN4 , UQOR1

Background

The mitochondrial respiratory chain provides energy to cells via oxidative phosphorylation and consists of four membrane-bound electron-transporting protein complexes (I-IV) and an ATP synthase (complex V). This gene encodes a 51 kDa subunit of the NADH:ubiquinone oxidoreductase complex I; a large complex with at least 45 nuclear and mitochondrial encoded subunits that liberates electrons from NADH and channels them to ubiquinone. This subunit carries the NADH-binding site as well as flavin mononucleotide (FMN)- and Fe-S-biding sites. Defects in complex I are a common cause of mitochondrial dysfunction; a syndrome that occurs in approximately 1 in 10,000 live births. Mitochondrial complex I deficiency is linked to myopathies, encephalomyopathies, and neurodegenerative disorders such as Parkinson's disease and Leigh syndrome. Alternative splicing results in multiple transcript variants encoding distinct isoforms.[provided by RefSeq, Oct 2009]

Database

Research Area

DATA IMAGES

Anti-NDUFV1 antibody [HL1600] used in Western Blot (WB). GTX637079

GTX637079 WB Image

KO/KD

Wild-type (WT) and NDUFV1 knockout (KO) 293T cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with NDUFV1 antibody [HL1600] (GTX637079) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-NDUFV1 antibody [HL1600] used in IHC (Paraffin sections) (IHC-P). GTX637079

GTX637079 IHC-P Image

NDUFV1 antibody [HL1600] detects NDUFV1 protein by immunohistochemical analysis.
Sample: Paraffin-embedded rat tissues.
NDUFV1 stained by NDUFV1 antibody [HL1600] (GTX637079) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-NDUFV1 antibody [HL1600] used in Western Blot (WB). GTX637079

GTX637079 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with NDUFV1 antibody [HL1600] (GTX637079) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

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Package List Price ($)
$ 399
$ 169
Custom Conjugations