GeneTex
United States (US)

NDUFV2 antibody

GTX102259_WB_19011506_957.jpg
GTX102259_39694_20141219_IHC_M_19011506_630.jpg
GTX102259_39694_WB_M_liver_19011506_650.jpg
GTX102259_39694_20141219_IHC_M_2_19011506_646.jpg
GTX102259_39694_WB_R_liver_19011506_496.jpg

Cat No. GTX102259

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

27 kDa. ( Note )

Positive Control

A431 , mouse liver , rat liver

Predict Reactivity

Zebrafish, Bovine, Pig, Chimpanzee, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.44 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human NDUFV2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Nadh:Ubiquinone Oxidoreductase Core Subunit V2,Ci-24K,Ndufv2

Cellular Localization

Mitochondrion inner membrane

Background

The NADH-ubiquinone oxidoreductase complex (complex I) of the mitochondrial respiratory chain catalyzes the transfer of electrons from NADH to ubiquinone, and consists of at least 43 subunits. The complex is located in the inner mitochondrial membrane. This gene encodes the 24 kDa subunit of complex I, and is involved in electron transfer. Mutations in this gene are implicated in Parkinson's disease, bipolar disorder, schizophrenia, and have been found in one case of early onset hypertrophic cardiomyopathy and encephalopathy. A non-transcribed pseudogene of this locus is found on chromosome 19. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX102259_WB_19011506_957.jpg

GTX102259 WB Image

Sample(30 μg of whole cell lysate)
A:A431(GTX27909)
12% SDS PAGE
GTX102259 diluted at 1:1500

GTX102259_39694_20141219_IHC_M_19011506_630.jpg

GTX102259 IHC-P Image

NDUFV2 antibody detects NDUFV2 protein at mitochondria on mouse fore brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse fore brain.
NDUFV2 antibody (GTX102259) diluted at 1:1000.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX102259_39694_WB_M_liver_19011506_650.jpg

GTX102259 WB Image

NDUFV2 antibody detects NDUFV2 protein by Western blot analysis.
A. 50 µg mouse liver lysate/extract
12 % SDS-PAGE
NDUFV2 antibody (GTX102259) dilution: 1:500

GTX102259_39694_20141219_IHC_M_2_19011506_646.jpg

GTX102259 IHC-P Image

NDUFV2 antibody detects NDUFV2 protein at mitochondria on mouse heart by immunohistochemical analysis.
Sample: Paraffin-embedded mouse heart.
NDUFV2 antibody (GTX102259) diluted at 1:1000.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX102259_39694_WB_R_liver_19011506_496.jpg

GTX102259 WB Image

NDUFV2 antibody detects NDUFV2 protein by Western blot analysis.
A. 50 µg rat liver lysate/extract
12 % SDS-PAGE
NDUFV2 antibody (GTX102259) dilution: 1:500

REFERENCE
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169