APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:3000 |
| 1:100-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
mouse liver , rat liver , A431
Predict Reactivity
Zebrafish, Bovine, Pig, Chimpanzee, Rhesus Monkey(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS, 10% Glycerol
Preservative
0.01% Thimerosal
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.44 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human NDUFV2. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_1241097
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
NADH:ubiquinone oxidoreductase core subunit V2 , CI-24k , MC1DN7
Cellular Localization
Mitochondrion inner membrane
Background
The NADH-ubiquinone oxidoreductase complex (complex I) of the mitochondrial respiratory chain catalyzes the transfer of electrons from NADH to ubiquinone, and consists of at least 43 subunits. The complex is located in the inner mitochondrial membrane. This gene encodes the 24 kDa subunit of complex I, and is involved in electron transfer. Mutations in this gene are implicated in Parkinson's disease, bipolar disorder, schizophrenia, and have been found in one case of early onset hypertrophic cardiomyopathy and encephalopathy. A non-transcribed pseudogene of this locus is found on chromosome 19. [provided by RefSeq]
Database
Research Area
DATA IMAGES
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GTX102259 WB Image
NDUFV2 antibody detects NDUFV2 protein by Western blot analysis.
A. 50 μg mouse liver lysate/extract
12 % SDS-PAGE
NDUFV2 antibody (GTX102259) dilution: 1:500
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GTX102259 IHC-P Image
NDUFV2 antibody detects NDUFV2 protein at mitochondria on mouse heart by immunohistochemical analysis.
Sample: Paraffin-embedded mouse heart.
NDUFV2 antibody (GTX102259) diluted at 1:1000.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX102259 WB Image
Sample(30 μg of whole cell lysate)
A:A431(GTX27909)
12% SDS PAGE
GTX102259 diluted at 1:1500
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GTX102259 IHC-P Image
NDUFV2 antibody detects NDUFV2 protein at mitochondria on mouse heart by immunohistochemical analysis.
Sample: Paraffin-embedded mouse heart.
NDUFV2 antibody (GTX102259) diluted at 1:1000.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
|
|
|
GTX102259 WB Image
NDUFV2 antibody detects NDUFV2 protein by Western blot analysis.
A. 50 μg rat liver lysate/extract
12 % SDS-PAGE
NDUFV2 antibody (GTX102259) dilution: 1:500
|
REFERENCE
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