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NECAB3 antibody

Anti-NECAB3 antibody used in Western Blot (WB). GTX87171
Anti-NECAB3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX87171

Cat. No. GTX87171

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human
Package
100 μg ($399)
See all NECAB3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500~1:1000
ICC/IF 1:100~1:500
Not tested in other applications.

Calculated MW

44 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS (without Mg²⁺ and Ca²⁺) pH7.4, 150mM NaCl, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The antiserum was produced against synthesized peptide derived from human NECAB3 (321-370).

Purification

Purified by antigen-affinity chromatography
From serum

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

APBA2BP , EFCBP3 , NECAB3 , NIP1 , Nterminal EFhand calcium binding protein 3 , STIP3 , SYTIP2 , XB51 , dJ63M2.4 , dJ63M2.5 , N-terminal EF-hand calcium binding protein 3

Cellular Localization

Golgi apparatus

Background

The protein encoded by this gene interacts with the amino-terminal domain of the neuron-specific X11-like protein (X11L), inhibits the association of X11L with amyloid precursor protein through a non-competitive mechanism, and abolishes the suppression of beta-amyloid production by X11L. This protein, together with X11L, may play an important role in the regulatory system of amyloid precursor protein metabolism and beta-amyloid generation. The protein is phosphorylated by NIMA-related expressed kinase 2, and localizes to the Golgi apparatus. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-NECAB3 antibody used in Western Blot (WB). GTX87171

GTX87171 WB Image

WB analysis of HepG2, COLO, and HT-29 cell lysates using GTX87171 NECAB3 antibody. The lane on the right is blocked with the synthesized peptide.

Anti-NECAB3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX87171

GTX87171 ICC/IF Image

ICC/IF analysis of A549 cells using GTX87171 NECAB3 antibody. The picture on the right is blocked with the synthesized peptide.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399