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NEDD4 antibody, Internal

Anti-NEDD4 antibody, Internal used in Western Blot (WB). GTX46935
Anti-NEDD4 antibody, Internal used in Western Blot (WB). GTX46935

Cat. No. GTX46935

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB

Reactivity

 Human, Mouse
Package
50 μg ($399)
See all NEDD4 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 0.2-2.5 ug/ml
Not tested in other applications.

Calculated MW

149 kDa. ( Note )

Positive Control

MCF-7 , mouse adipose tissue

PROPERTIES

Form

Liquid

Buffer

PBS, 2% Sucrose

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.5-1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthetic peptide corresponding to an Internal region of Human NEDD4

Purification

Affinity Purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

neural precursor cell expressed, developmentally down-regulated 4, E3 ubiquitin protein ligase , NEDD4-1 , RPF1

Cellular Localization

Cytoplasm,Cell membrane

Background

This gene is the founding member of the NEDD4 family of HECT ubiquitin ligases that function in the ubiquitin proteasome system of protein degradation. The encoded protein contains an N-terminal calcium and phospholipid binding C2 domain followed by multiple tryptophan-rich WW domains and, a C-terminal HECT ubiquitin ligase catalytic domain. It plays critical role in the regulation of a number of membrane receptors, endocytic machinery components and the tumor suppressor PTEN. [provided by RefSeq, Jul 2016]

Database

Research Area

DATA IMAGES

Anti-NEDD4 antibody, Internal used in Western Blot (WB). GTX46935

GTX46935 WB Image

WB analysis of MCF-7 cells using GTX46935 NEDD4 antibody at 0.2-1μg/ml.

Anti-NEDD4 antibody, Internal used in Western Blot (WB). GTX46935

GTX46935 WB Image

WB analysis of mouse adipose tissue using GTX46935 NEDD4 antibody at 1:1000.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399