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NEDD4 antibody

Anti-NEDD4 antibody used in Western Blot (WB). GTX32745
Anti-NEDD4 antibody used in IHC (Paraffin sections) (IHC-P). GTX32745
Anti-NEDD4 antibody used in IHC (Paraffin sections) (IHC-P). GTX32745

Cat. No. GTX32745

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human
Package
50 μl ($399)
See all NEDD4 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
IHC-P 1:50 - 1:200
Not tested in other applications.

Calculated MW

149 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 160-460 of human NEDD4 (NP_940682.2).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

neural precursor cell expressed, developmentally down-regulated 4, E3 ubiquitin protein ligase , NEDD4-1 , RPF1

Cellular Localization

Cytoplasm,Cell membrane

Background

This gene is the founding member of the NEDD4 family of HECT ubiquitin ligases that function in the ubiquitin proteasome system of protein degradation. The encoded protein contains an N-terminal calcium and phospholipid binding C2 domain followed by multiple tryptophan-rich WW domains and, a C-terminal HECT ubiquitin ligase catalytic domain. It plays critical role in the regulation of a number of membrane receptors, endocytic machinery components and the tumor suppressor PTEN. [provided by RefSeq, Jul 2016]

Database

Research Area

DATA IMAGES

Anti-NEDD4 antibody used in Western Blot (WB). GTX32745

GTX32745 WB Image

WB analysis of various sample lysates using GTX32745 NEDD4 antibody. The signal was developed with ECL plus-Enhanced.
Dilution : 1:1000
Loading : 25μg per lane

Anti-NEDD4 antibody used in IHC (Paraffin sections) (IHC-P). GTX32745

GTX32745 IHC-P Image

IHC-P analysis of human gastric tissue using GTX32745 NEDD4 antibody.
Dilution : 1:200

Anti-NEDD4 antibody used in IHC (Paraffin sections) (IHC-P). GTX32745

GTX32745 IHC-P Image

IHC-P analysis of human prostate tissue using GTX32745 NEDD4 antibody.
Dilution : 1:200

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399