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NEDD4L antibody, Internal

Anti-NEDD4L antibody, Internal used in Western Blot (WB). GTX46924
Anti-NEDD4L antibody, Internal used in Western Blot (WB). GTX46924

Cat. No. GTX46924

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB

Reactivity

 Human
Package
50 μg ($409)
See all NEDD4L products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 0.2-2.5 ug/ml
Not tested in other applications.

Calculated MW

112 kDa. ( Note )

Positive Control

cystic fibrosis epithelial cells , PANC1

PROPERTIES

Form

Liquid

Buffer

PBS, 2% Sucrose

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.5-1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthetic peptide corresponding to an Internal region of Human NEDD4L

Purification

Affinity Purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase , NEDD4-2 , NEDD4.2 , PVNH7 , RSP5 , hNEDD4-2

Cellular Localization

Cytoplasm,Golgi apparatus

Background

This gene encodes a member of the Nedd4 family of HECT domain E3 ubiquitin ligases. HECT domain E3 ubiquitin ligases transfer ubiquitin from E2 ubiquitin-conjugating enzymes to protein substrates, thus targeting specific proteins for lysosomal degradation. The encoded protein mediates the ubiquitination of multiple target substrates and plays a critical role in epithelial sodium transport by regulating the cell surface expression of the epithelial sodium channel, ENaC. Single nucleotide polymorphisms in this gene may be associated with essential hypertension. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Mar 2012]

Database

Research Area

DATA IMAGES

Anti-NEDD4L antibody, Internal used in Western Blot (WB). GTX46924

GTX46924 WB Image

WB analysis of human cystic fibrosis epithelial cells using GTX46924 NEDD4L antibody at 1:3000.

Anti-NEDD4L antibody, Internal used in Western Blot (WB). GTX46924

GTX46924 WB Image

WB analysis of PANC1 cells using GTX46924 NEDD4L antibody at 0.2-1μg/ml.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 409