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NEDD4L antibody

Anti-NEDD4L antibody used in Western Blot (WB). GTX130730

Cat. No. GTX130730

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all NEDD4L products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
Not tested in other applications.

Calculated MW

112 kDa. ( Note )

Positive Control

PC-3

Predict Reactivity

Mouse, Rat, Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.53 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human NEDD4L. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2886330

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase , NEDD4-2 , NEDD4.2 , PVNH7 , RSP5 , hNEDD4-2

Cellular Localization

Cytoplasm

Database

Research Area

DATA IMAGES

Anti-NEDD4L antibody used in Western Blot (WB). GTX130730

GTX130730 WB Image

NEDD4L antibody detects NEDD4L protein by western blot analysis. Whole cell extracts (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with NEDD4L antibody (GTX130730) diluted at 1:1000.

REFERENCE

Application Reference

Chen KC et al. PLoS One 2019; 14 (12) : e0225913 IGF-1-enhanced miR-513a-5p signaling desensitizes glioma cells to?temozolomide?by targeting the NEDD4L-inhibited Wnt/帣-catenin?pathway.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169