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NEDD8 antibody [5B8]

Anti-NEDD8 antibody [5B8] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX60546
Anti-NEDD8 antibody [5B8] used in ELISA (ELISA). GTX60546
Anti-NEDD8 antibody [5B8] used in Western Blot (WB). GTX60546
Anti-NEDD8 antibody [5B8] used in Flow cytometry (FACS). GTX60546
Anti-NEDD8 antibody [5B8] used in Western Blot (WB). GTX60546
Anti-NEDD8 antibody [5B8] used in IHC (Paraffin sections) (IHC-P). GTX60546

Cat. No. GTX60546

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 5B8

Isotype

 IgG1

Application

 WB, ICC/IF, IHC-P, FACS, ELISA

Reactivity

 Human, Rat
Package
100 μg ($409)
See all NEDD8 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1/500 - 1/2000
ICC/IF 1/200 - 1/1000
IHC-P 1/200 - 1/1000
FACS 1/200 - 1/400
ELISA 1/10000
Not tested in other applications.

Calculated MW

9 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Purified recombinant fragment of human NEDD8 expressed in E. Coli.

Purification

Protein G Purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

neural precursor cell expressed, developmentally down-regulated 8 , NEDD-8

Cellular Localization

Nucleus

Database

Research Area

DATA IMAGES

Anti-NEDD8 antibody [5B8] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX60546

GTX60546 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX60546 NEDD8 antibody [5B8].
Green : NEDD8
Red: Actin filaments

Anti-NEDD8 antibody [5B8] used in ELISA (ELISA). GTX60546

GTX60546 ELISA Image

ELISA analysis of antigen using GTX60546 NEDD8 antibody [5B8].
Black : Control antigen 100ng
Purple : Antigen 10ng
Blue : Antigen 50ng
Red : Antigen 100ng

Anti-NEDD8 antibody [5B8] used in Western Blot (WB). GTX60546

GTX60546 WB Image

WB analysis of human NEDD8 (AA: 1-81) recombinant protein using GTX60546 NEDD8 antibody [5B8].

Anti-NEDD8 antibody [5B8] used in Flow cytometry (FACS). GTX60546

GTX60546 FACS Image

FACS analysis of HeLa cells using GTX60546 NEDD8 antibody [5B8].
Green : NEDD8
Red : negative control

Anti-NEDD8 antibody [5B8] used in Western Blot (WB). GTX60546

GTX60546 WB Image

WB analysis of C6 (1) and HeLa (2) cell lysate using GTX60546 NEDD8 antibody [5B8].

Anti-NEDD8 antibody [5B8] used in IHC (Paraffin sections) (IHC-P). GTX60546

GTX60546 IHC-P Image

IHC-P analysis of prostate cancer tissue using GTX60546 NEDD8 antibody [5B8].

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 409