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NEIL1 antibody [N1C1]

Anti-NEIL1 antibody [N1C1] used in Western Blot (WB). GTX100102

Cat No. GTX100102

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB
Reactivity Human
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
Not tested in other applications.

Calculated MW

44 kDa. ( Note )

Positive Control

human NEIL1-transfected 293T

Predict Reactivity

Mouse, Rat, Bovine(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.36 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human NEIL1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

nei like DNA glycosylase 1 , FPG1 , NEI1 , hFPG1

Cellular Localization

Nucleus

Background

NEIL1 belongs to a class of DNA glycosylases homologous to the bacterial Fpg/Nei family. These glycosylases initiate the first step in base excision repair by cleaving bases damaged by reactive oxygen species and introducing a DNA strand break via the associated lyase reaction (Bandaru et al., 2002 [PubMed 12509226]).[supplied by OMIM]

Database

Research Area

DATA IMAGES
Anti-NEIL1 antibody [N1C1] used in Western Blot (WB). GTX100102

GTX100102 WB Image

NEIL1 antibody [N1C1] detects NEIL1 protein by western blot analysis. Non-transfected (-) and NEIL1-transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with NEIL1 antibody [N1C1] (GTX100102) diluted at 1:500.

REFERENCE
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REVIEW
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SDS
Glycerol.pdf
Thimerosal.pdf
Document
Unmodified.pdf