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NEK2 antibody [1B6]

Anti-NEK2 antibody [1B6] used in Western Blot (WB). GTX70281
Anti-NEK2 antibody [1B6] used in Western Blot (WB). GTX70281

Cat. No. GTX70281

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 1B6

Isotype

 IgG1

Application

 WB

Reactivity

 Human
Package
100 μl ($399)
See all NEK2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:1000

Note :

WB
It detects both isoforms 1 & 2 in Jurkat lysate - see image online.
Not tested in other applications.

Calculated MW

52 kDa. ( Note )

Positive Control

Jurkat cell lysate

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.43 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide Ð Immunogen sequence information is commercially sensitive

Purification

Protein G purified

Conjugation

Unconjugated

RRID

AB_1080971

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

NIMA related kinase 2 , HsPK21 , NEK2A , NLK1 , PPP1R111 , RP67

Cellular Localization

Cytoplasm , Nucleolus

Background

This gene encodes a serine/threonine-protein kinase that is involved in mitotic regulation. This protein is localized to the centrosome, and undetectable during G1 phase, but accumulates progressively throughout the S phase, reaching maximal levels in late G2 phase. Alternatively spliced transcript variants encoding different isoforms with distinct C-termini have been noted for this gene. [provided by RefSeq, Feb 2011]

Database

Research Area

DATA IMAGES

Anti-NEK2 antibody [1B6] used in Western Blot (WB). GTX70281

GTX70281 WB Image

Detection of human NEK2 protein using GeneTex NEK2 1B6 monoclonal antibody (GTX70281) in Jurkat whole cell extract.
Anti-NEK2 antibody [1B6] used in Western Blot (WB). GTX70281

GTX70281 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with NEK2 antibody [1B6] (GTX70281) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Package List Price ($)
$ 399