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NEK2 antibody

Orthogonal
Anti-NEK2 antibody used in IHC (Paraffin sections) (IHC-P). GTX118735
Anti-NEK2 antibody used in Western Blot (WB). GTX118735
Anti-NEK2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX118735
Anti-NEK2 antibody used in Western Blot (WB). GTX118735

Cat. No. GTX118735

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all NEK2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

52 kDa. ( Note )

Positive Control

293T

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.47 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein  corresponding to human NEK2.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10630077

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

NIMA related kinase 2 , HsPK21 , NEK2A , NLK1 , PPP1R111 , RP67

Cellular Localization

Nucleus , Isoform 1: Nucleus , nucleolus , Isoform 2: Cytoplasm

Background

Protein kinase that is involved in mitotic regulation. Integral component of the mitotic spindle-assembly checkpoint which is necessary for proper chromosome segregation during metaphase-anaphase transition. Required for association of MAD2L1 to kinetochore. Phosphorylates SGOL1. May have a role at the G2-M transition. May also play a role in meiosis. Isoform 1 but not isoform 2 appears to play a role in centrosome splitting. Isoform 1 phosphorylates and activates NEK11 in G1/S-arrested cells. Isoform 2, which is not present in the nucleolus, does not.

Database

Research Area

DATA IMAGES

Anti-NEK2 antibody used in IHC (Paraffin sections) (IHC-P). GTX118735

GTX118735 IHC-P Image

NEK2 antibody detects NEK2 protein at cytoplasm and nucleus on human ovarian carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human ovarian carcinoma.
NEK2 antibody (GTX118735) diluted at 1:250.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-NEK2 antibody used in Western Blot (WB). GTX118735

GTX118735 WB Image

Sample (30 ug of whole cell lysate)
A: 293T
10% SDS PAGE
GTX118735 diluted at 1:1000

Anti-NEK2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX118735

GTX118735 ICC/IF Image

Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using NEK2(GTX118735) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.

Anti-NEK2 antibody used in Western Blot (WB). GTX118735

GTX118735 WB Image

Orthogonal

Untreated (–) and treated (+) HeLa whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with NEK2 antibody (GTX118735) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169