GeneTex
United States (US)

NEK4 antibody [N2C1], Internal

GTX102826_WB_19011506_681.jpg
GTX102826_40086_IFA_19011506_409.jpg
GTX102826_40086_20180615_WB_19011506_862.jpg
GTX102826_IHC_19011506_514.jpg

Cat No. GTX102826

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

95 kDa. ( Note )

Positive Control

A431 , H1299 , U87-MG , SK-N-SH , IMR32 , SK-N-AS
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.37 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human NEK4. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Nima Related Kinase 4,Nrk2,Stk2,Pp12301,Nek4

Cellular Localization

Nucleus

Database

Research Area

DATA IMAGES
GTX102826_WB_19011506_681.jpg

GTX102826 WB Image

Sample (30 ug of whole cell lysate)
A: A431 (GTX27909)
B: H1299
7.5% SDS PAGE
GTX102826 diluted at 1:1000

GTX102826_40086_IFA_19011506_409.jpg

GTX102826 ICC/IF Image

NEK4 antibody [N2C1], Internal detects NEK4 protein at nucleus by confocal immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 15 min.
Green: NEK4 protein stained by NEK4 antibody [N2C1], Internal (GTX102826) diluted at 1:500.
Blue: Hoechst 33343 staining.
[Images captured by Olympus FV10i Confocal Laser Scanning Microscope]

GTX102826_40086_20180615_WB_19011506_862.jpg

GTX102826 WB Image

Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with NEK4 antibody [N2C1], Internal (GTX102826) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX102826_IHC_19011506_514.jpg

GTX102826 IHC-P Image

Immunohistochemical analysis of paraffin-embedded A549 Xenograft, using NEK4(GTX102826) antibody at 1:100 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169