Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with NEK4 antibody [N2C1], Internal (GTX102826) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

NEK4 antibody [N2C1], Internal detects NEK4 protein at nucleus by confocal immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 15 min.
Green: NEK4 protein stained by NEK4 antibody [N2C1], Internal (GTX102826) diluted at 1:500.
Blue: Hoechst 33343 staining.
[Images captured by Olympus FV10i Confocal Laser Scanning Microscope]

Sample (30 ug of whole cell lysate)
A: A431 (GTX27909)
B: H1299
7.5% SDS PAGE
GTX102826 diluted at 1:1000

Immunohistochemical analysis of paraffin-embedded A549 Xenograft, using NEK4(GTX102826) antibody at 1:100 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min