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NEK4 antibody [N2C1], Internal

Anti-NEK4 antibody [N2C1], Internal used in IHC (Paraffin sections) (IHC-P). GTX102826
Anti-NEK4 antibody [N2C1], Internal used in Western Blot (WB). GTX102826
Anti-NEK4 antibody [N2C1], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX102826
Anti-NEK4 antibody [N2C1], Internal used in Western Blot (WB). GTX102826

Cat. No. GTX102826

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all NEK4 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

95 kDa. ( Note )

Positive Control

A431 , H1299 , U87-MG , SK-N-SH , IMR32 , SK-N-AS

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.37 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human NEK4. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950988

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

NIMA related kinase 4 , NRK2 , STK2 , pp12301

Cellular Localization

Nucleus

Database

Research Area

DATA IMAGES

Anti-NEK4 antibody [N2C1], Internal used in IHC (Paraffin sections) (IHC-P). GTX102826

GTX102826 IHC-P Image

Immunohistochemical analysis of paraffin-embedded A549 Xenograft, using NEK4(GTX102826) antibody at 1:100 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-NEK4 antibody [N2C1], Internal used in Western Blot (WB). GTX102826

GTX102826 WB Image

Sample (30 ug of whole cell lysate)
A: A431 (GTX27909)
B: H1299
7.5% SDS PAGE
GTX102826 diluted at 1:1000
Anti-NEK4 antibody [N2C1], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX102826

GTX102826 ICC/IF Image

NEK4 antibody [N2C1], Internal detects NEK4 protein at nucleus by confocal immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 15 min.
Green: NEK4 protein stained by NEK4 antibody [N2C1], Internal (GTX102826) diluted at 1:500.
Blue: Hoechst 33343 staining.
[Images captured by Olympus FV10i Confocal Laser Scanning Microscope]

Anti-NEK4 antibody [N2C1], Internal used in Western Blot (WB). GTX102826

GTX102826 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with NEK4 antibody [N2C1], Internal (GTX102826) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169