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NEK6 antibody [5D7]

Anti-NEK6 antibody [5D7] used in Flow cytometry (FACS). GTX84058
Anti-NEK6 antibody [5D7] used in IHC (Paraffin sections) (IHC-P). GTX84058
Anti-NEK6 antibody [5D7] used in IHC (Paraffin sections) (IHC-P). GTX84058
Anti-NEK6 antibody [5D7] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84058
Anti-NEK6 antibody [5D7] used in IHC (Paraffin sections) (IHC-P). GTX84058
Anti-NEK6 antibody [5D7] used in Western Blot (WB). GTX84058
Anti-NEK6 antibody [5D7] used in IHC (Paraffin sections) (IHC-P). GTX84058
Anti-NEK6 antibody [5D7] used in IHC (Paraffin sections) (IHC-P). GTX84058
Anti-NEK6 antibody [5D7] used in IHC (Paraffin sections) (IHC-P). GTX84058
Anti-NEK6 antibody [5D7] used in IHC (Paraffin sections) (IHC-P). GTX84058
Anti-NEK6 antibody [5D7] used in IHC (Paraffin sections) (IHC-P). GTX84058

Cat. No. GTX84058

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 5D7

Isotype

 IgG1

Application

 WB, ICC/IF, IHC-P, FACS

Reactivity

 Human
Package
100 μl ($519)
See all NEK6 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:1000
ICC/IF 1:100
IHC-P 1:50
FACS 1:100
Not tested in other applications.

Calculated MW

36 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.75 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human recombinant protein of human NEK6 (NP_055212) produced in HEK293T cell.

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

NIMA related kinase 6 , SID6-1512

Cellular Localization

Cytoplasm,Nucleus,Nucleus speckle

Background

Activated during M phase. Required for chromosome segregation at metaphase-anaphase transition and therefore for mitotic progression. Inhibition of activity results in apoptosis. Phosphorylates KIF11 to promote mitotic spindle formation.

Database

Research Area

DATA IMAGES

Anti-NEK6 antibody [5D7] used in Flow cytometry (FACS). GTX84058

GTX84058 FACS Image

FACS analysis of HEK293T cells transfected with either NEK6 plasmid(Red) or empty vector control plasmid(Blue) using GTX84058 NEK6 antibody [5D7].

Anti-NEK6 antibody [5D7] used in IHC (Paraffin sections) (IHC-P). GTX84058

GTX84058 IHC-P Image

IHC-P analysis of human breast adenocarcinoma tissue using GTX84058 NEK6 antibody [5D7].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

Anti-NEK6 antibody [5D7] used in IHC (Paraffin sections) (IHC-P). GTX84058

GTX84058 IHC-P Image

IHC-P analysis of human colon adenocarcinoma tissue using GTX84058 NEK6 antibody [5D7].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

Anti-NEK6 antibody [5D7] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84058

GTX84058 ICC/IF Image

ICC/IF analysis of COS7 cells transiently transfected with NEK6 plasmid using GTX84058 NEK6 antibody [5D7].

Anti-NEK6 antibody [5D7] used in IHC (Paraffin sections) (IHC-P). GTX84058

GTX84058 IHC-P Image

IHC-P analysis of human thyroid carcinoma tissue using GTX84058 NEK6 antibody [5D7].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

Anti-NEK6 antibody [5D7] used in Western Blot (WB). GTX84058

GTX84058 WB Image

WB analysis of HEK293T cells transfected with NEK6 plasmid (Right) or empty vector (Left) for 48 hrs using GTX84058 NEK6 antibody [5D7].
Loading : 5 ug per lane

Anti-NEK6 antibody [5D7] used in IHC (Paraffin sections) (IHC-P). GTX84058

GTX84058 IHC-P Image

IHC-P analysis of human lung carcinoma tissue using GTX84058 NEK6 antibody [5D7].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

Anti-NEK6 antibody [5D7] used in IHC (Paraffin sections) (IHC-P). GTX84058

GTX84058 IHC-P Image

IHC-P analysis of human ovary adenocarcinoma tissue using GTX84058 NEK6 antibody [5D7].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

Anti-NEK6 antibody [5D7] used in IHC (Paraffin sections) (IHC-P). GTX84058

GTX84058 IHC-P Image

IHC-P analysis of human pancreas carcinoma tissue using GTX84058 NEK6 antibody [5D7].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

Anti-NEK6 antibody [5D7] used in IHC (Paraffin sections) (IHC-P). GTX84058

GTX84058 IHC-P Image

IHC-P analysis of human prostate carcinoma tissue using GTX84058 NEK6 antibody [5D7].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

Anti-NEK6 antibody [5D7] used in IHC (Paraffin sections) (IHC-P). GTX84058

GTX84058 IHC-P Image

IHC-P analysis of human kidney carcinoma tissue using GTX84058 NEK6 antibody [5D7].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 519