IP analysis of DDDDK tagged NEK6 overexpressed HEK293T lysate using GTX84056 NEK6 antibody [8G5].After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDDDK polyclonal antibody.
IP reaction : 2μg antibody / 500μl cell lysate

IHC-P analysis of breast adenocarcinoma tissue using GTX84056 NEK6 antibody [8G5].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

IHC-P analysis of pancreas carcinoma tissue using GTX84056 NEK6 antibody [8G5].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

IHC-P analysis of endometrium tissue using GTX84056 NEK6 antibody [8G5].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

IHC-P analysis of kidney carcinoma tissue using GTX84056 NEK6 antibody [8G5].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

ICC/IF analysis of COS7 cells transiently transfected with NEK6 plasmid using GTX84056 NEK6 antibody [8G5].

WB analysis of HEK293T cells transfected with NEK6 plasmid (Right) or empty vector (Left) for 48 hrs using GTX84056 NEK6 antibody [8G5].
Loading : 5 ug per lane

IHC-P analysis of ovary tissue using GTX84056 NEK6 antibody [8G5].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50