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NEK6 antibody [8G5]

Anti-NEK6 antibody [8G5] used in IHC (Paraffin sections) (IHC-P). GTX84056
Anti-NEK6 antibody [8G5] used in Western Blot (WB). GTX84056
Anti-NEK6 antibody [8G5] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84056
Anti-NEK6 antibody [8G5] used in IHC (Paraffin sections) (IHC-P). GTX84056
Anti-NEK6 antibody [8G5] used in IHC (Paraffin sections) (IHC-P). GTX84056
Anti-NEK6 antibody [8G5] used in IHC (Paraffin sections) (IHC-P). GTX84056
Anti-NEK6 antibody [8G5] used in IHC (Paraffin sections) (IHC-P). GTX84056
Anti-NEK6 antibody [8G5] used in Immunoprecipitation (IP). GTX84056

Cat. No. GTX84056

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 8G5

Isotype

 IgG1

Application

 WB, ICC/IF, IHC-P, IP

Reactivity

 Human
Package
100 μl ($519)
See all NEK6 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500
ICC/IF 1:100
IHC-P 1:50
IP 2-4ug/mg
Not tested in other applications.

Calculated MW

36 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.74 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full-length protein expressed in 293T cell transfected with human NEK6 expression vector

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

NIMA related kinase 6 , SID6-1512

Cellular Localization

Cytoplasm,Nucleus,Nucleus speckle

Background

Activated during M phase. Required for chromosome segregation at metaphase-anaphase transition and therefore for mitotic progression. Inhibition of activity results in apoptosis. Phosphorylates KIF11 to promote mitotic spindle formation.

Database

Research Area

DATA IMAGES

Anti-NEK6 antibody [8G5] used in IHC (Paraffin sections) (IHC-P). GTX84056

GTX84056 IHC-P Image

IHC-P analysis of ovary tissue using GTX84056 NEK6 antibody [8G5].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

Anti-NEK6 antibody [8G5] used in Western Blot (WB). GTX84056

GTX84056 WB Image

WB analysis of HEK293T cells transfected with NEK6 plasmid (Right) or empty vector (Left) for 48 hrs using GTX84056 NEK6 antibody [8G5].
Loading : 5 ug per lane

Anti-NEK6 antibody [8G5] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84056

GTX84056 ICC/IF Image

ICC/IF analysis of COS7 cells transiently transfected with NEK6 plasmid using GTX84056 NEK6 antibody [8G5].

Anti-NEK6 antibody [8G5] used in IHC (Paraffin sections) (IHC-P). GTX84056

GTX84056 IHC-P Image

IHC-P analysis of kidney carcinoma tissue using GTX84056 NEK6 antibody [8G5].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

Anti-NEK6 antibody [8G5] used in IHC (Paraffin sections) (IHC-P). GTX84056

GTX84056 IHC-P Image

IHC-P analysis of endometrium tissue using GTX84056 NEK6 antibody [8G5].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

Anti-NEK6 antibody [8G5] used in IHC (Paraffin sections) (IHC-P). GTX84056

GTX84056 IHC-P Image

IHC-P analysis of pancreas carcinoma tissue using GTX84056 NEK6 antibody [8G5].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

Anti-NEK6 antibody [8G5] used in IHC (Paraffin sections) (IHC-P). GTX84056

GTX84056 IHC-P Image

IHC-P analysis of breast adenocarcinoma tissue using GTX84056 NEK6 antibody [8G5].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

Anti-NEK6 antibody [8G5] used in Immunoprecipitation (IP). GTX84056

GTX84056 IP Image

IP analysis of DDDDK tagged NEK6 overexpressed HEK293T lysate using GTX84056 NEK6 antibody [8G5].After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDDDK polyclonal antibody.
IP reaction : 2μg antibody / 500μl cell lysate

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 519