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NEK7 antibody [C2C3], C-term

Anti-NEK7 antibody [C2C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX108216
Anti-NEK7 antibody [C2C3], C-term used in Western Blot (WB). GTX108216
Anti-NEK7 antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX108216

Cat No. GTX108216

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

35 kDa. ( Note )

Positive Control

HeLaS3

Predict Reactivity

Rat, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.85 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human NEK7. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Nima Related Kinase 7,Nek7

Cellular Localization

Cytoplasm

Background

NIMA-related kinases share high amino acid sequence identity with the gene product of the Aspergillus nidulans 'never in mitosis A' gene, which controls initiation of mitosis.[supplied by OMIM]

Database

DATA IMAGES
Anti-NEK7 antibody [C2C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX108216

GTX108216 ICC/IF Image

NEK7 antibody [C2C3], C-term detects NEK7 protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: NEK7 protein stained by NEK7 antibody [C2C3], C-term (GTX108216) diluted at 1:200.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Anti-NEK7 antibody [C2C3], C-term used in Western Blot (WB). GTX108216

GTX108216 WB Image

Sample(30 μg of whole cell lysate)
A:HeLa S3(GTX14654)
12% SDS PAGE
GTX108216 diluted at 1:500

Anti-NEK7 antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX108216

GTX108216 IHC-P Image

NEK7 antibody [C2C3], C-term detects NEK7 protein at cytoplasm on mouse muscle by immunohistochemical analysis.
Sample: Paraffin-embedded mouse muscle.
NEK7 antibody [C2C3], C-term (GTX108216) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE
There are currently no references for NEK7 antibody [C2C3], C-term (GTX108216). Be the first to share your publications with this product.
REVIEW
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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169