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NEK7 antibody [HL1348]

Anti-NEK7 antibody [HL1348] used in Western Blot (WB). GTX636768
Anti-NEK7 antibody [HL1348] used in IHC (Paraffin sections) (IHC-P). GTX636768
Anti-NEK7 antibody [HL1348] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636768
Anti-NEK7 antibody [HL1348] used in Western Blot (WB). GTX636768
Anti-NEK7 antibody [HL1348] used in IHC (Paraffin sections) (IHC-P). GTX636768

Cat. No. GTX636768

Host

Rabbit

Clonality

Monoclonal

Clone Name

HL1348

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human, Mouse
Package
100 μl ($399),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

35 kDa. ( Note )

Predict Reactivity

Rat, Chicken, Xenopus tropicalis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human NEK7. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

RRID

AB_2910072

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

NIMA related kinase 7

Cellular Localization

Nucleus,Cytoplasm

Background

NIMA-related kinases share high amino acid sequence identity with the gene product of the Aspergillus nidulans 'never in mitosis A' gene, which controls initiation of mitosis.[supplied by OMIM, Jul 2002]

Database

Research Area

DATA IMAGES

Anti-NEK7 antibody [HL1348] used in Western Blot (WB). GTX636768

GTX636768 WB Image

Wild-type (WT) and NEK7 knockout (KO) HeLa cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with NEK7 antibody [HL1348] (GTX636768) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-NEK7 antibody [HL1348] used in IHC (Paraffin sections) (IHC-P). GTX636768

GTX636768 IHC-P Image

NEK7 antibody [HL1348] detects NEK7 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse heart.
NEK7 stained by NEK7 antibody [HL1348] (GTX636768) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-NEK7 antibody [HL1348] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636768

GTX636768 ICC/IF Image

NEK7 antibody [HL1348] detects NEK7 protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: NEK7 stained by NEK7 antibody [HL1348] (GTX636768) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-NEK7 antibody [HL1348] used in Western Blot (WB). GTX636768

GTX636768 WB Image

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with NEK7 antibody [HL1348] (GTX636768) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-NEK7 antibody [HL1348] used in IHC (Paraffin sections) (IHC-P). GTX636768

GTX636768 IHC-P Image

NEK7 antibody [HL1348] detects NEK7 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse muscle.
NEK7 stained by NEK7 antibody [HL1348] (GTX636768) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

There are currently no references for NEK7 antibody [HL1348] (GTX636768). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for NEK7 antibody [HL1348] (GTX636768). Be the first to share your experience with this product.
Package List Price ($)
$ 399
$ 169
Custom Conjugations