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NELF-E antibody

Anti-NELF-E antibody used in Western Blot (WB). GTX33353
Anti-NELF-E antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX33353

Cat No. GTX33353

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF

Reactivity

Human
Package
100 μl ($329)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:100
Not tested in other applications.

Calculated MW

43 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS pH7.3, 0.02% sodium azide, 50% glycerol.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human NELFE (NP_002895.3).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

negative elongation factor complex member E , D6S45 , NELF-E , RD , RDBP , RDP

Cellular Localization

Nucleus

Background

The protein encoded by this gene is part of a complex termed negative elongation factor (NELF) which represses RNA polymerase II transcript elongation. This protein bears similarity to nuclear RNA-binding proteins; however, it has not been demonstrated that this protein binds RNA. The protein contains a tract of alternating basic and acidic residues, largely arginine (R) and aspartic acid (D). The gene localizes to the major histocompatibility complex (MHC) class III region on chromosome 6. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-NELF-E antibody used in Western Blot (WB). GTX33353

GTX33353 WB Image

WB analysis of various samples using GTX33353 NELF-E antibody.
Dilution : 1:1000
Loading : 25μg per lane

Anti-NELF-E antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX33353

GTX33353 ICC/IF Image

ICC/IF analysis of U2OS cell using GTX33353 NELF-E antibody.

REFERENCE

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REVIEW

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Package List Price ($)
$ 329