APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1 - 2 μg/mL |
| 5 μg/mL |
| Assay dependent |
Not tested in other applications.
Calculated MW
Positive Control
HeLa Cell Lysate
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Rabbit polyclonal NELF antibody was raised against a 17 amino acid peptide near the center of human NELF.The immunogen is located within amino acids 230 - 280 of NELF.
Purification
Purified by antigen-affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
NMDA receptor synaptonuclear signaling and neuronal migration factor , HH9 , NELF
Cellular Localization
Nucleus,Cytoplasm,Cytoplasm, cytoskeleton,Cell membrane,Cell projection, dendrite,Cell junction, synapse,Membrane
Background
The protein encoded by this gene is involved in guidance of olfactory axon projections and migration of luteinizing hormone-releasing hormone neurons. Defects in this gene are a cause of idiopathic hypogonadotropic hypogonadism (IHH). Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2010]
Database
Research Area
DATA IMAGES
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GTX31872 IHC-P Image
IHC-P analysis of rat brain tissue using GTX31872 NELF antibody.
Working concentration : 20 μg/ml
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GTX31872 WB Image
WB analysis of HeLa cell lysate using GTX31872 NELF antibody.
Working concentration : (A) 1 and (B) 2 μg/ml
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GTX31872 IHC-P Image
IHC-P analysis of rat brain tissue using GTX31872 NELF antibody.
Working concentration : 2.5 μg/ml
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GTX31872 IHC-P Image
IHC-P analysis of mouse brain tissue using GTX31872 NELF antibody.
Working concentration : 5 μg/ml
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GTX31872 IHC-P Image
IHC-P analysis of mouse brain tissue using GTX31872 NELF antibody.
Working concentration : 20 μg/ml
Green : Primary antibody
Blue : DAPI
Red : Actin
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REFERENCE
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REVIEW
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