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NELL1 antibody [C1C3]

Anti-NELL1 antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX111493

Cat. No. GTX111493

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 IHC-P, IHC

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all NELL1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
IHC-P Assay dependent
IHC Assay dependent
Not tested in other applications.

Calculated MW

90 kDa. ( Note )

Predict Reactivity

Mouse, Rat(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human NELL1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10617837

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

neural EGFL like 1 , IDH3GL , NRP1

Cellular Localization

Secreted

Background

This gene encodes a cytoplasmic protein that contains epidermal growth factor (EGF)-like repeats. The encoded heterotrimeric protein may be involved in cell growth regulation and differentiation. A similar protein in rodents is involved in craniosynostosis. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-NELL1 antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX111493

GTX111493 IHC-P Image

NELL1 antibody [C1C3] detects NELL1 protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
NELL1 stained by NELL1 antibody [C1C3] (GTX111493) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

Application Reference

Shen J et al. J Orthop 2015; 12 (Suppl 2) : S223-9 NELL-1 expression in tumors of cartilage.
Application : IHC-P
Reactivity : Human
Shen J et al. Biochem Biophys Res Commun 2015; 460 (2) : 368-74 NELL-1 expression in benign and malignant bone tumors.
Application : IHC
Reactivity : Human
Submit a Reference

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169