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NELL1 antibody

Anti-NELL1 antibody used in Western Blot (WB). GTX103819
Anti-NELL1 antibody used in Western Blot (WB). GTX103819
Anti-NELL1 antibody used in Western Blot (WB). GTX103819
Anti-NELL1 antibody used in IHC (Paraffin sections) (IHC-P). GTX103819

Cat No. GTX103819

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($329),
25 μl ($169)
See all NELL1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:10000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

90 kDa. ( Note )

Positive Control

NT2D1 , IMR32

PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.58 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human NELL1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

neural EGFL like 1 , IDH3GL , NRP1

Cellular Localization

Secreted

Background

This gene encodes a cytoplasmic protein that contains epidermal growth factor (EGF)-like repeats. The encoded heterotrimeric protein may be involved in cell growth regulation and differentiation. A similar protein in rodents is involved in craniosynostosis. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-NELL1 antibody used in Western Blot (WB). GTX103819

GTX103819 WB Image

Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with NELL1 antibody (GTX103819) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-NELL1 antibody used in Western Blot (WB). GTX103819

GTX103819 WB Image

Various tissue extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with NELL1 antibody (GTX103819) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-NELL1 antibody used in Western Blot (WB). GTX103819

GTX103819 WB Image

Sample (30 ug of whole cell lysate)
A: NT2D1
B: IMR32
7.5% SDS PAGE
GTX103819 diluted at 1:5000

Anti-NELL1 antibody used in IHC (Paraffin sections) (IHC-P). GTX103819

GTX103819 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human breast cancer, using NELL1(GTX103819) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

Application Reference

Chen X et al. Cell Death Differ 2019; (Epub) Cumulative inactivation of Nell-1 in Wnt1 expressing cell lineages results in craniofacial skeletal hypoplasia and postnatal hydrocephalus.
Application : WB
Reactivity : Mouse
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 329
$ 169