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NENF antibody

Anti-NENF antibody used in IHC (Paraffin sections) (IHC-P). GTX85028
Anti-NENF antibody used in Western Blot (WB). GTX85028
Anti-NENF antibody used in IHC (Paraffin sections) (IHC-P). GTX85028

Cat. No. GTX85028

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, ELISA

Reactivity

 Human, Mouse, Rat
Package
100 μg ($399)
See all NENF products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1 - 2 μg/mL
IHC-P 10 μg/mL
ELISA Assay dependent
Not tested in other applications.

Calculated MW

19 kDa. ( Note )

Positive Control

Human Small Intestine Tissue Lysate(GTX27923)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

NENF antibody was raised against a 15 amino acid synthetic peptide from near the carboxy terminus of human NENF.The immunogen is located within the last 50 amino acids of NENF.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

neudesin neurotrophic factor , CIR2 , SCIRP10 , SPUF

Background

NENF is a secreted protein that is expressed in neurons but not glial cells of the brain. This protein has neurotrophic activity in primary cultured mouse neurons but not mitogenic activity in primary cultured mouse astrocytes. NENF activated mitogen-activated protein (MAP) and phosphotidylinositol-3 kinase pathways and could be inhibited by pertussis toxin but not receptor tyrosine kinases, suggesting that NENF acts through a Gi/Go-protein-coupled receptor. Despite its predicted molecular weight of ~20kDa, NENF is observed at 37kDa in SDS-PAGE.

Database

Research Area

DATA IMAGES

Anti-NENF antibody used in IHC (Paraffin sections) (IHC-P). GTX85028

GTX85028 IHC-P Image

IHC-P analysis of rat kidney tissue using GTX85028 NENF antibody.
Working concentration : 10 μg/ml

Anti-NENF antibody used in Western Blot (WB). GTX85028

GTX85028 WB Image

WB analysis of human kidney tissue lysate using GTX85028 NENF antibody.
Working concentration : (A) 1 and (B) 2 μg/ml

Anti-NENF antibody used in IHC (Paraffin sections) (IHC-P). GTX85028

GTX85028 IHC-P Image

IHC-P analysis of rat kidney tissue using GTX85028 NENF antibody.
Working concentration : 20 μg/ml

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399