APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 2.5μg/ml |
|
Note :
IHC-P
Human Breast shows cytoplasm staining of myoepithelial cells of ducts.
|
Not tested in other applications.
Positive Control
The peptide used to generate this antibody is available for purchase (GTX89335-PEP).
Product Note
This antibody is expected to recognize both reported isoforms (NP_001040625.1 and NP_005854.2).
Predict Reactivity
Mouse, Rat, Dog(>80% identity)
PROPERTIES
Form
Liquid
Buffer
TBS, 0.5% BSA
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.50 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Peptide with sequence C-DKLEYLDEKQRDPR, from the internal region of the protein sequence according to NP_005854.2; NP_001040625.1.
Purification
Purified by ammonium sulphate precipitation followed by antigen affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
neuroepithelial cell transforming 1 , ARHGEF8 , NET1A
Cellular Localization
Cytoplasm , Nucleus
Background
This gene is part of the family of Rho guanine nucleotide exchange factors. Members of this family activate Rho proteins by catalyzing the exchange of GDP for GTP. The protein encoded by this gene interacts with RhoA within the cell nucleus and may play a role in repairing DNA damage after ionizing radiation. Pseudogenes of this gene are located on the long arms of chromosomes 1, 7 and 18. Alternative splicing results in multiple transcript variants that encode different protein isoforms. [provided by RefSeq, Jul 2012]
Database
Research Area
DATA IMAGES
|
GTX89335 IHC-P Image
IHC-P analysis of human breast using GTX89335 NET1 antibody, Internal.
Antigen retrieval : citrate buffer pH 6
Dilution : 2.5μg/ml
|
REFERENCE
There are currently no references for NET1 antibody, Internal (GTX89335). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for NET1 antibody, Internal (GTX89335). Be the first to share your experience with this product.
