Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Dilution |
| 1:500-1:3000 |
| 1:100-1:1000 |
| Assay dependent |
| Assay dependent |
|
Note :
target validated by Mass Spectrometry
|
Not tested in other applications.
Calculated MW
Positive Control
293T , mouse spleen
Predict Reactivity
Rat, Bovine, Pig, Rhesus Monkey(>80% identity)
Form
Liquid
Buffer
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1.03mg/ml
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human NF-E2. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Synonyms
NF-E2 antibody, p45 antibody, NFE2 antibody, leucine zipper protein NF-E2 antibody, transcription factor NF-E2 45 kDa subunit antibody, "nuclear factor, erythroid-derived 2 45 kDa subunit antibody", p45 NF-E2 antibody, "nuclear factor (erythroid-derived 2), 45kDa antibody"
Cellular Localization
Nucleus
Background
Component of the NF-E2 complex essential for regulating erythroid and megakaryocytic maturation and differentiation. Binds to the hypersensitive site 2 (HS2) of the beta-globin control region (LCR). This subunit (NFE2) recognizes the TCAT/C sequence of the AP-1-like core palindrome present in a number of erythroid and megakaryocytic gene promoters. Requires MAFK or other small MAF proteins for binding to the NF-E2 motif. May play a role in all aspects of hemoglobin production from globin and heme synthesis to procurement of iron.
Database
