GeneTex
United States (US)

NF-E2 antibody [N1C3]

GTX102698_39946_WB_M_spleen_19011506_636.jpg
GTX102698_WB_19011506_306.jpg
GTX102698_39946_IFA_19011506_918.jpg

Cat No. GTX102698

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IP, ChIP-seq
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IP Assay dependent
ChIP-seq Assay dependent

Note :

target validated by Mass Spectrometry
Not tested in other applications.

Calculated MW

41 kDa. ( Note )

Positive Control

293T , mouse spleen

Predict Reactivity

Rat, Bovine, Pig, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.03mg/ml

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human NF-E2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

NF-E2 antibody, p45 antibody, NFE2 antibody, leucine zipper protein NF-E2 antibody, transcription factor NF-E2 45 kDa subunit antibody, "nuclear factor, erythroid-derived 2 45 kDa subunit antibody", p45 NF-E2 antibody, "nuclear factor (erythroid-derived 2), 45kDa antibody"

Cellular Localization

Nucleus

Background

Component of the NF-E2 complex essential for regulating erythroid and megakaryocytic maturation and differentiation. Binds to the hypersensitive site 2 (HS2) of the beta-globin control region (LCR). This subunit (NFE2) recognizes the TCAT/C sequence of the AP-1-like core palindrome present in a number of erythroid and megakaryocytic gene promoters. Requires MAFK or other small MAF proteins for binding to the NF-E2 motif. May play a role in all aspects of hemoglobin production from globin and heme synthesis to procurement of iron.
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Database

DATA IMAGES
GTX102698_39946_WB_M_spleen_19011506_636.jpg

GTX102698 WB Image

Sample (50 µg of whole cell lysate)
A: mouse spleen
10% SDS PAGE
GTX102698 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX102698_WB_19011506_306.jpg

GTX102698 WB Image

Sample (30 µg of whole cell lysate)
A: 293T
10% SDS PAGE
GTX102698 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX102698_39946_IFA_19011506_918.jpg

GTX102698 ICC/IF Image

NF-E2 antibody [N1C3] detects NF-E2 protein at nucleus by immunofluorescent analysis.
Sample: HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: NF-E2 protein stained by NF-E2 antibody [N1C3] (GTX102698) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Reference
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169