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NF-H antibody [8H10]

Anti-NF-H antibody [8H10] used in IHC (Paraffin sections) (IHC-P). GTX60650
Anti-NF-H antibody [8H10] used in ELISA (ELISA). GTX60650
Anti-NF-H antibody [8H10] used in Western Blot (WB). GTX60650

Cat. No. GTX60650

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 8H10

Isotype

 IgG1

Application

 WB, IHC-P, ELISA

Reactivity

 Human
Package
100 μg ($409)
See all NF-H products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1/500 - 1/2000
IHC-P 1/200 - 1/1000
ELISA 1/10000
Not tested in other applications.

Calculated MW

112 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Purified recombinant fragment of human NF-H (AA: 968-1020) expressed in E. Coli.

Purification

Protein G Purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

neurofilament heavy , CMT2CC , NFH

Cellular Localization

Cytoplasm

Background

Neurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and functionally maintain neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. This gene encodes the heavy neurofilament protein. This protein is commonly used as a biomarker of neuronal damage and susceptibility to amyotrophic lateral sclerosis (ALS) has been associated with mutations in this gene. [provided by RefSeq, Oct 2008]

Database

Research Area

DATA IMAGES

Anti-NF-H antibody [8H10] used in IHC (Paraffin sections) (IHC-P). GTX60650

GTX60650 IHC-P Image

IHC-P analysis of human medulla oblongata tissue using GTX60650 NF-H antibody [8H10].

Anti-NF-H antibody [8H10] used in ELISA (ELISA). GTX60650

GTX60650 ELISA Image

ELISA analysis of antigen using GTX60650 NF-H antibody [8H10].
Black : Control antigen 100ng
Purple : Antigen 10ng
Blue : Antigen 50ng
Red : Antigen 100ng

Anti-NF-H antibody [8H10] used in Western Blot (WB). GTX60650

GTX60650 WB Image

WB analysis of HEK293 (1) and NF-H (AA: 968-1020)-hIgGFc transfected HEK293 (2) cell lysate using GTX60650 NF-H antibody [8H10].

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 409