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NF-H antibody [NF421]

Anti-NF-H antibody [NF421] used in IHC (Paraffin sections) (IHC-P). GTX34897
Anti-NF-H antibody [NF421] used in IHC (Paraffin sections) (IHC-P). GTX34897

Cat No. GTX34897

Host Mouse
Clonality Monoclonal
Clone Name NF421
Isotype IgG1
Application IHC-P
Reactivity Human, Mouse, Rat, Chicken, Pig
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
IHC-P 0.25-0.5ug/ml for 30 minutes at RT

Note :

IHC-P
Boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes

Not tested in other applications.

Calculated MW

112 kDa. ( Note )

Specificity/Sensitivity

This MAb reacts with a 200kDa protein, identified as heavy sub-unit of neurofilaments (NF-H). Neurofilaments make up the main structural elements of axons and dendrites and are found in neurons, peripheral nerves, and sympathetic ganglion cells. Neurofilaments consist of three major subunits with molecular weights of 68kDa (NF-L), 160kDa (NF-M) and 200kDa (NF-H). Anti-neurofilament stains a number of neural, neuroendocrine, and endocrine tumors. Neuromas, ganglioneuromas, gangliogliomas, ganglioneuroblastomas, and neuroblastomas stain positively for anti-neurofilament. Neurofilaments are also present in paragangliomas as well as adrenal and extra-adrenal pheochromocytomas. Carcinoids, neuroendocrine carcinomas of the skin, and oat cell carcinomas of the lung also express neurofilament.
PROPERTIES

Form

Liquid

Buffer

Prepared in 10mM PBS with 0.05% BSA and 0.05% azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Human recombinant neurofilament protein

Purification

Ab purified from Bioreactor Concentrate by Protein A/G

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Neurofilament Heavy,Cmt2Cc,Nfh,Nefh

Background

Neurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and functionally maintain neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. This gene encodes the heavy neurofilament protein. This protein is commonly used as a biomarker of neuronal damage and susceptibility to amyotrophic lateral sclerosis (ALS) has been associated with mutations in this gene. [provided by RefSeq, Oct 2008]

Database

Research Area

DATA IMAGES
Anti-NF-H antibody [NF421] used in IHC (Paraffin sections) (IHC-P). GTX34897

GTX34897 IHC-P Image

Formalin-fixed, paraffin-embedded human Cerebellum stained with Neurofilament Monoclonal Antibody (NF421).

Anti-NF-H antibody [NF421] used in IHC (Paraffin sections) (IHC-P). GTX34897

GTX34897 IHC-P Image

Formalin-fixed, paraffin-embedded Rat Cerebellum stained with Neurofilament Monoclonal Antibody (NF421).

Package List Price ($)
$ 329