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NF-H antibody [NF421]

Anti-NF-H antibody [NF421] used in Flow cytometry (FACS). GTX34897
Anti-NF-H antibody [NF421] used in IHC (Paraffin sections) (IHC-P). GTX34897
Anti-NF-H antibody [NF421] used in IHC (Paraffin sections) (IHC-P). GTX34897
Anti-NF-H antibody [NF421] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX34897

Cat. No. GTX34897

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 NF421

Isotype

 IgG1

Application

 ICC/IF, IHC-P, FACS

Reactivity

 Human, Mouse, Rat, Chicken, Pig
Package
100 μg ($529)
See all NF-H products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
ICC/IF 1-2μg/ml
IHC-P 0.25-0.5μg/ml for 30 minutes at RT
FACS 1-2μg/10⁶ cells

Note :

IHC-P
Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95ºC followed by cooling at RT for 20 minutes.
Not tested in other applications.

Calculated MW

112 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

10mM PBS, 0.05% BSA

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant full-length human neurofilament (NEFH) protein

Purification

Protein A/G purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

CMT2CC , NEFH , NFH , neurofilament heavy polypeptide , Neurofilament NF-H , NF-200 , NF200 , neurofilament heavy , 200 kD Neurofilament Heavy

Cellular Localization

Cytoplasm

Background

Neurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and functionally maintain neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. This gene encodes the heavy neurofilament protein. This protein is commonly used as a biomarker of neuronal damage and susceptibility to amyotrophic lateral sclerosis (ALS) has been associated with mutations in this gene. [provided by RefSeq, Oct 2008]

Database

Research Area

DATA IMAGES

Anti-NF-H antibody [NF421] used in Flow cytometry (FACS). GTX34897

GTX34897 FACS Image

FACS analysis of HEK293 cells using GTX34897 NF-H antibody [NF421].
Blue : Primary antibody
Red : Isotype control

Anti-NF-H antibody [NF421] used in IHC (Paraffin sections) (IHC-P). GTX34897

GTX34897 IHC-P Image

IHC-P analysis of human cerebellum tissue using GTX34897 NF-H antibody [NF421].

Anti-NF-H antibody [NF421] used in IHC (Paraffin sections) (IHC-P). GTX34897

GTX34897 IHC-P Image

IHC-P analysis of rat cerebellum tissue using GTX34897 NF-H antibody [NF421].

Anti-NF-H antibody [NF421] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX34897

GTX34897 ICC/IF Image

ICC/IF analysis of HEK293 cells using GTX34897 NF-H antibody [NF421].
Green : Primary antibody
Red : Phalloidin

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 529