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NF-H antibody [RT97]

Cat No. GTX74884

Reference 1

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 RT97

Isotype

 IgG1

Application

 IHC-P, IHC-Fr, IHC

Reactivity

 Human, Mouse, Rat, Chicken, Pig
Package
40 μg ($219)
See all NF-H products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
IHC-P 1/50-1/100
IHC-Fr Assay dependent
IHC Assay dependent
Not tested in other applications.

Positive Control

Brain or spinal cord

Specificity/Sensitivity

GTX74884 recognises the 200kD neurofilament protein in a range of species. The antibody stains various tumours including phaeochromocytoma, paraganglioma and ganglioneuroblastoma._x000B_It has also been demonstrated that GTX74884 also recognises a phosphorylation dependent epitope on microtubule associated protein 1B (MAP1B) and on Tau in Western blots. Reactivity of the antibody with MAP1B and Tau is not seen under the conditions for immunohistochemistry.

PROPERTIES

Form

Liquid

Buffer

PBS, 0.09% Sodium Azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.0 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Rat

Immunogen

Triton X-100-insoluble rat brain protein.

Purification

Purified by hydrophobic interaction chromatography
From tissue culture supernatant

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Neurofilament Heavy , Nfh , Nefh

Research Area

REFERENCE

Application Reference

Takiguchi M et al. IBRO Reports 2018; 4 : 43106 Neonatal spinal injury induces de novo projections of primary afferents to the lumbosacral intermediolateral nucleus in rats.
Application : IHC
Reactivity : Rat
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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 219