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NF-H antibody

Anti-NF-H antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX110065
Anti-NF-H antibody used in IHC (Frozen sections) (IHC-Fr). GTX110065
Anti-NF-H antibody used in IHC (Frozen sections) (IHC-Fr). GTX110065
Anti-NF-H antibody used in IHC (Paraffin sections) (IHC-P). GTX110065
Anti-NF-H antibody used in IHC (Paraffin sections) (IHC-P). GTX110065
Anti-NF-H antibody used in Western Blot (WB). GTX110065
Anti-NF-H antibody used in IHC (Frozen sections) (IHC-Fr). GTX110065
Anti-NF-H antibody used in Western Blot (WB). GTX110065

Cat. No. GTX110065

Reference 3

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IHC-Fr

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all NF-H products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr 1:100-1:1000
Not tested in other applications.

Calculated MW

112 kDa. ( Note )

Positive Control

rat brain , IMR32

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.71 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human NF-H. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950985

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

neurofilament heavy , CMT2CC , NFH

Background

Neurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and functionally maintain neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. This gene encodes the heavy neurofilament protein. This protein is commonly used as a biomarker of neuronal damage and susceptibility to amyotrophic lateral sclerosis (ALS) has been associated with mutations in this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-NF-H antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX110065

GTX110065 ICC/IF Image

NF-H antibody detects NF-H protein at cytoplasm by immunofluorescent analysis.
Sample: DIV9 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min.
Green: NF-H protein stained by NF-H antibody (GTX110065) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Anti-NF-H antibody used in IHC (Frozen sections) (IHC-Fr). GTX110065

GTX110065 IHC-Fr Image

NF-H antibody detects NF-H protein expression by immunohistochemical analysis.
Sample: Frozen sectioned E13.5 Rat brain.
Green: NF-H protein stained by NF-H antibody (GTX110065) diluted at 1:250.
Red: beta Tubulin 3/ TUJ1, a mature neuron marker, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Anti-NF-H antibody used in IHC (Frozen sections) (IHC-Fr). GTX110065

GTX110065 IHC-Fr Image

NF-H antibody detects NF-H protein expression by immunohistochemical analysis.
Sample: Frozen-sectioned adult mouse cerebellum.
Green: NF-H protein stained by NF-H antibody (GTX110065) diluted at 1:250.
Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Anti-NF-H antibody used in IHC (Paraffin sections) (IHC-P). GTX110065

GTX110065 IHC-P Image

NF-H antibody detects NF-H protein expression by immunohistochemical analysis.
Sample:Paraffin-Embedded adult mouse retina.
Green: NF-H protein stained by NF-H antibody (GTX110065) diluted at 1:250.
Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Anti-NF-H antibody used in IHC (Paraffin sections) (IHC-P). GTX110065

GTX110065 IHC-P Image

NF-H antibody detects NF-H protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse spinal cord.
NF-H stained by NF-H antibody (GTX110065) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-NF-H antibody used in Western Blot (WB). GTX110065

GTX110065 WB Image

Whole cell extract (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with NF-H antibody (GTX110065) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-NF-H antibody used in IHC (Frozen sections) (IHC-Fr). GTX110065

GTX110065 IHC-Fr Image

NF-H antibody detects NF-H protein expression by immunohistochemical analysis.
Sample: Frozen-sectioned adult mouse cerebellum.
Green: NF-H protein stained by NF-H antibody (GTX110065) diluted at 1:250.
Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Anti-NF-H antibody used in Western Blot (WB). GTX110065

GTX110065 WB Image

Rat tissue extract (50 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with NF-H antibody (GTX110065) diluted at 1:500.

REFERENCE

Application Reference

Treffy RW et al. Dev Cell 2021; 56 (19) : 2752-2764.e6 Neuroblastoma differentiation in?vivo excludes cranial tumors.
Tomita Y et al. Biochem Biophys Res Commun 2019; 519 (3) : 462-468 Ninjurin 1 mediates peripheral nerve regeneration through Schwann cell maturation of NG2-positive cells.
Pigna E et al. EBioMedicine 2019; 40 : 717-732 Histone deacetylase 4 protects from denervation and skeletal muscle atrophy in a murine model of amyotrophic lateral sclerosis.
Application : IHC-Fr
Reactivity : Mouse
Submit a Reference

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169