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NF-H antibody

Anti-NF-H antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX85459

Cat. No. GTX85459

Host

 Chicken

Clonality

 Polyclonal

Isotype

 IgY

Application

 WB, ICC/IF, IHC

Reactivity

 Human, Mouse, Rat
Package
50 μg ($399)
See all NF-H products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:2000-1:5000
ICC/IF Assay dependent
IHC 1:2000-1:5000
Not tested in other applications.

Calculated MW

112 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS pH7.2, 1% BSA

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Bovine

Immunogen

Chickens were immunized with NF-H protein purified from adult bovine brains.

Purification

IgY fraction

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

neurofilament heavy , CMT2CC , NFH

Cellular Localization

Cytoplasm

Background

Neurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and functionally maintain neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. This gene encodes the heavy neurofilament protein. This protein is commonly used as a biomarker of neuronal damage and susceptibility to amyotrophic lateral sclerosis (ALS) has been associated with mutations in this gene. [provided by RefSeq, Oct 2008]

Database

Research Area

DATA IMAGES

Anti-NF-H antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX85459

GTX85459 ICC/IF Image

ICC/IF analysis of cortical neuron from a neo-natal mouse brain culture using GTX85459 NF-H antibody.
Dilution : 1:5000

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399