APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1/500 - 1/2000 |
| 1/200 - 1/1000 |
| 1/200 - 1/1000 |
| 1/200 - 1/400 |
| 1/10000 |
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
Ascites
Preservative
0.03% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Antigen Species
Human
Immunogen
Purified recombinant fragment of human NEFL expressed in E. Coli.
Purification
Unpurified
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
neurofilament light , CMT1F , CMT2E , CMTDIG , NF-L , NF68 , NFL , PPP1R110
Background
Neurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and they functionally maintain the neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. This gene encodes the light chain neurofilament protein. Mutations in this gene cause Charcot-Marie-Tooth disease types 1F (CMT1F) and 2E (CMT2E), disorders of the peripheral nervous system that are characterized by distinct neuropathies. A pseudogene has been identified on chromosome Y. [provided by RefSeq, Oct 2008]
Database
Research Area
DATA IMAGES
![Anti-NF-L antibody [1H3] used in Western Blot (WB). GTX60541](/templates/default/js/lightbox/images/loading.gif "Anti-NF-L antibody [1H3] used in Western Blot (WB). GTX60541")
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GTX60541 WB Image
WB analysis of human NF-L (AA: 422-543) recombinant protein using GTX60541 NF-L antibody [1H3].
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GTX60541 ELISA Image
ELISA analysis of antigen using GTX60541 NF-L antibody [1H3].
Black : Control antigen 100ng
Purple : Antigen 10ng
Blue : Antigen 50ng
Red : Antigen 100ng
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GTX60541 WB Image
WB analysis of HeLa (1) and Jurkat (2) cell lysate using GTX60541 NF-L antibody [1H3].
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GTX60541 ICC/IF Image
ICC/IF analysis of HeLa cells using GTX60541 NF-L antibody [1H3].
Green : NF-L
Blue: DRAQ5 fluorescent DNA dye
Red: Actin filaments
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GTX60541 FACS Image
FACS analysis of Jurkat cells using GTX60541 NF-L antibody [1H3].
Green : NF-L
Red : negative control
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GTX60541 IHC-P Image
IHC-P analysis of lung cancer tissue using GTX60541 NF-L antibody [1H3].
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GTX60541 IHC-P Image
IHC-P analysis of bladder cancer tissue using GTX60541 NF-L antibody [1H3].
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REFERENCE
There are currently no references for NF-L antibody [1H3] (GTX60541). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for NF-L antibody [1H3] (GTX60541). Be the first to share your experience with this product.
![Anti-NF-L antibody [1H3] used in Western Blot (WB). GTX60541](/upload/website/prouct_img/small/GTX60541/GTX60541_20170912_WB_1_w_23061123_627.webp "Anti-NF-L antibody [1H3] used in Western Blot (WB). GTX60541")
![Anti-NF-L antibody [1H3] used in ELISA (ELISA). GTX60541](/upload/website/prouct_img/small/GTX60541/GTX60541_20170912_ELISA_w_23061123_125.webp "Anti-NF-L antibody [1H3] used in ELISA (ELISA). GTX60541")
![Anti-NF-L antibody [1H3] used in Western Blot (WB). GTX60541](/upload/website/prouct_img/small/GTX60541/GTX60541_20170912_WB_w_23061123_588.webp "Anti-NF-L antibody [1H3] used in Western Blot (WB). GTX60541")
![Anti-NF-L antibody [1H3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX60541](/upload/website/prouct_img/small/GTX60541/GTX60541_20170912_ICCIF_w_23061123_692.webp "Anti-NF-L antibody [1H3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX60541")
![Anti-NF-L antibody [1H3] used in Flow cytometry (FACS). GTX60541](/upload/website/prouct_img/small/GTX60541/GTX60541_20170912_FACS_w_23061123_112.webp "Anti-NF-L antibody [1H3] used in Flow cytometry (FACS). GTX60541")
![Anti-NF-L antibody [1H3] used in IHC (Paraffin sections) (IHC-P). GTX60541](/upload/website/prouct_img/small/GTX60541/GTX60541_20170912_IHC-P_1_w_23061123_483.webp "Anti-NF-L antibody [1H3] used in IHC (Paraffin sections) (IHC-P). GTX60541")
![Anti-NF-L antibody [1H3] used in IHC (Paraffin sections) (IHC-P). GTX60541](/upload/website/prouct_img/small/GTX60541/GTX60541_20170912_IHC-P_w_23061123_342.webp "Anti-NF-L antibody [1H3] used in IHC (Paraffin sections) (IHC-P). GTX60541")
![Anti-NF-L antibody [1H3] used in Western Blot (WB). GTX60541](/upload/website/prouct_img/normal/GTX60541/GTX60541_20170912_WB_1_w_23061123_627.webp "Anti-NF-L antibody [1H3] used in Western Blot (WB). GTX60541")
![Anti-NF-L antibody [1H3] used in ELISA (ELISA). GTX60541](/upload/website/prouct_img/normal/GTX60541/GTX60541_20170912_ELISA_w_23061123_125.webp "Anti-NF-L antibody [1H3] used in ELISA (ELISA). GTX60541")
![Anti-NF-L antibody [1H3] used in Western Blot (WB). GTX60541](/upload/website/prouct_img/normal/GTX60541/GTX60541_20170912_WB_w_23061123_588.webp "Anti-NF-L antibody [1H3] used in Western Blot (WB). GTX60541")
![Anti-NF-L antibody [1H3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX60541](/upload/website/prouct_img/normal/GTX60541/GTX60541_20170912_ICCIF_w_23061123_692.webp "Anti-NF-L antibody [1H3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX60541")
![Anti-NF-L antibody [1H3] used in Flow cytometry (FACS). GTX60541](/upload/website/prouct_img/normal/GTX60541/GTX60541_20170912_FACS_w_23061123_112.webp "Anti-NF-L antibody [1H3] used in Flow cytometry (FACS). GTX60541")
![Anti-NF-L antibody [1H3] used in IHC (Paraffin sections) (IHC-P). GTX60541](/upload/website/prouct_img/normal/GTX60541/GTX60541_20170912_IHC-P_1_w_23061123_483.webp "Anti-NF-L antibody [1H3] used in IHC (Paraffin sections) (IHC-P). GTX60541")
![Anti-NF-L antibody [1H3] used in IHC (Paraffin sections) (IHC-P). GTX60541](/upload/website/prouct_img/normal/GTX60541/GTX60541_20170912_IHC-P_w_23061123_342.webp "Anti-NF-L antibody [1H3] used in IHC (Paraffin sections) (IHC-P). GTX60541")